| Perinereis aibuhitensis belongs to Annelids，Polychaete，Nereidida，Nereidae，Perinereis. It has become a emerging aquaculture object with a very optimisticoutlook in industrialization due to its good adaptbility, high nourishment value, highpopulation annual turnover rate and good economic returns, etc. However, due to itsunique breeding habits, the special culture conditions and the technology in artificialbreeding and farming is not yet stable. However, reports over the artificial breeding isfew due to its unique breeding habits, the special culture conditions, the unstabletechnology in artificial breeding and farming. And the best Culture sediment has notbeen reported.This paper studies the effects on the embryo development of the water salinity,pH and the egg density. Immediately explores the tolerance of the aibuhitensis larvaeto ammonia and sulfur and the survival rate and growth of the3-setiger nectochaeta indifferent sediment, salinity and density. Subsequently, study the survival rate andgrowth of the larva in different sediment and stocking density. Finally, artificialbreeding and culture technology of Perinereis aibuhitensis is described. The mainresults are summarized as follows:1. Salinity, pH and egg density have significant influences on the fertility andembryonic development of Perinereis aibuhitensis (P＜0.05), in the salinity of20,fertilization rate(FR) and hatching rate(HR) are both highest, namely84.13%and90.96%. FR and HR are the highest at pH7.5, FR is0at pH5.5, meanwhile the acidresistance is superior to the alkali resistance during the embryogenesis. When eggdensity is between20to40grain/mL, FR goes down when the egg density becomehigher, HR is around84%,no significant difference(P＞0.05).When the egg density is 50grain/mL, HR decreased significantly, and diapause at the multicellular stage.2. Acute toxicity test result shows that the effects of ammonia and sulphideconcentration on the survival rate of the3-setiger nectochaeta is significantly (p<0.05). The96h TLm of total ammonia-N and sulfid to3-setiger nectochaeta are2.42mg/L and0.43mg/L，the safe concentration(SC)are0.242mg/L and0.043mg/L.3. Salinity has a significant influence on the survival rate(SR)of thenectochaeta(p＜0.05).The SR is0in salinity5.In salinity20, the SR is94.21%, thehighest. Density has no significant influence on the SR and growth of the nectochaetawithin the test range(p＞0.05).Different sediments effect the SR and growth of thenectochaeta significantly(p＜0.05).4. When the blocking density is between1100to1700slips/m2,the survivalrate(SR) and growth of the nectochaeta are not different significantly(p＞0.05), andthey are significantly higher than the other density rows(p＜0.05).The SR and growthof the nectochaeta are not significantly different when the proportion ofsediment(mud:sand) are3:2and2:3(p＞0.05) and they are significantly higher thanother test rows(p＜0.05). Ammonia-N content in each group during the experiment isstable. But the sulfide obviously accumulates during the experiment and it accumulatesfaster during the test. Sulfide accumulates in rows of pure mud and80%mud faster,and deterioration of these two sets of sediment is most significant.In addition to these studies, the author participates in internships to grasp the keytechnologies of artificial breeding and farming. This part is analyzed as attached. |
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