| This experiment was conducted to study the effects of diet with high calcium and highcalcium-high protein on clinical pathololgy and related genes of chicken.18030-day-oldhealthy hy-line variety brown with similar conditions were chosen and randomly dividedinto three groups of60each. The growers in different groups were fed with normalcalcium and crude protein diet (1.00%calcium and17.53%crude protein, called groupⅠ), high calcium and normal protein diet (3.78%calcium and17.53%crude protein,called group Ⅱ) and high calcium and high crude protein diet (3.78%calcium and24.25%crude protein, called group Ⅲ). We collected venous blood samples from chicken’s wingand tussie samples from chicken’s liver and kidney on the30,40and50day after ourexperiment started. And this experiment was designed to research chicken’s productivity,blood routine, the function of kidney and liver, serum eletrolytes, the expression of relatedgenes through clinical pathology and Q-PCR. The results were as follows:(1) The diet with high calcium and high calcium-high protein resulted in the weight ofchicken’s body decreasing significantly (P<0.01), and the weight ratio of chicken’s kidneyand body increasing significantly (P<0.01).(2) Compared with group Ⅰ, RBC, HGB and PCV of group Ⅱ decreasedsignificantly (P<0.01), WBC and GRAN of group Ⅱ increased significantly (P<0.05);WBC, LYM, MID and GRAN of group Ⅲ increased compared with group Ⅰ, and theydecreased compared with group Ⅱ.(3) Compared with group Ⅰ, the concentration of serum Ca2+, Na+and Cl-of groupⅡ and Ⅲ increased significantly (P<0.01), the concentration of serum K+of group Ⅱand Ⅲ decreased significantly (P<0.05), and the concentration of serum inorganic P ofgroup Ⅱ and Ⅲ also decreased significantly (P<0.01).(4) Compared with group Ⅰ, the concentration of BUN, UA and TBIL and theactivity of GPT, GOT and5’-NT of group Ⅱ and Ⅲ increased significantly (P<0.05).(5) Compared with group Ⅰ, the activity of serum XOD increased significantly(P<0.01), the activity of serum SOD of group Ⅱ and Ⅲ decreased significantly(P<0.01), and the activity of serum GSH-Px of group Ⅱ and Ⅲ also decreasedsignificantly (P<0.05).(6) Compared with group Ⅰ, the expression of XOD, Bcl-2and Caspase3mRNA ofgroup Ⅱ and Ⅲ increased significantly (P<0.05).The results suggested that diet with high calcium and high calcium-high protein cancause a decline of hepatic and renal function and antioxidant function, but also causedrising of the activity of serum XOD; it also showed that high diet protein can causeimmune system weakened, as the same time it can be antagonistic to the lesion caused byhigh diet calcium. |
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