Study On Screening And Compound Synergistic Herbal Wood Preservative

Wood is a renewable, recyclable green materials, but mildew, discoloration and other limiting the use of wood. After the invasion of mold timber, so that the liquid permeability of the timber increases, can promote fungal infection eventually led to the timber blue stain. The study was conducted from wood fungicide screening10Chinese herbs selected, screened shamisen excellent herbal antifungal properties. Select acid, ACQ, moth mildew tablets three synergists, wood Aspergillus niger, discoloration bacteria (cocoa balls Diplodia) as test species, to determine the minimum inhibitory concentration of six agents. Liu Wei pharmaceutical compound alone and synergistic mechanism of the inhibition of fungal and antibacterial conducted a study to be non-wood molds in vitro test for the verification, provide basic data to support the further development and use of new wood and mildew theory.Test results are as follows:1. Select three kinds of herbal rugosa, orange peel, leaves, three kinds of synergist formic acid, ACQ, moth mildew tablets. Aspergillus niger wood, discolored bacteria (cocoa balls Diplodia) as test strains using PDA liquid culture method, microwave heating extraction method, the evaluation results using characterization methods mildew antibacterial effect, six agents were determined the minimum inhibitory concentration after drug synergism between composite made further study.2. Herbs and synergist on morphology of fungi. Select rugosa and ACQ effect on Aspergillus niger and discoloration bacteria, microscopic observation showed that after treatment rugosa ACQ and abnormal growth of Aspergillus niger, some hyphae appear tangled, twisted, and the mycelium was no significant abnormal constriction plump; discoloration bacteria mycelial colonies gradually thinning, increased branching hyphae and developmental deformity, adding agents on fungal morphology affected.3. Herbs, synergists and complex synergistic effect on mold growth cycle. Nephelometry results indicate that grows in the six kinds of poisonous agents in the medium and discoloration fungus Aspergillus niger, its growth curve significant changes have occurred. Fungus Aspergillus niger and discoloration under different growth curve pharmaceutical compound effect, indicating that the main agents inhibit mold growth log phase of growth, thus affecting their growth cycle.4. Mold and mildew inhibitory concentration lethal concentration of screening results indicate herbal rugosa, leaves, orange peel in turn lethal concentration increases; synergist moth mildew tablets, ACQ, in turn reducing the lethal concentration of formic acid. In vitro tests showed that non-timber PDA medium containing ACQ and hybrid rugosa to significantly inhibit the growth of bacteria effect, validate the results of the antibacterial properties of the first part.