The Research On The Optimization And Application Technology Of Mangroves PGPB Inoculum

Because of long-term human damage, mangrove forest resources reduced, forest degradation, protective effects decline in our country at present, Studies on PGPB in different environment, it is good for optimizing fungicide and providing the applied research for fungicide impact on plant, at the same time using PGPB inoculate casuarina seedlings, so it provides a guidance significance for PGPB inoculum to expand production and application.On the basis of previous studies, The experiment by optimizing combination the8nitrogen fixing strains and9phosphorus solubilizing strains, to select the best4phosphorus solubilizing strains and4nitrogen fixing strains for the best strains of composition and dosage forms development, develop the PGPB inoculation on mangrove and casuarina in various environments test. The results showed that:(1) This study selects the improved OAB and SRSM1medium for optimum combination,4phosphorus solubilizing strains P5, P7, B. lich, B. amy) and4nitrogen fixing strains (NGWB-yl, NLQQ-w14, A. halos, NGQQ-r14), producting and testing5combination bacteria, respectively ZH3(NGWB-yl+P7), ZH15(A. halos+B. lich), ZH5(NGQQ-r1+B. amy), ZH14(NGWB-yl+B. lich), ZH4(NLQQ-w14+P5). And develop for more than13bacteria strains to form three dosage forms of bacteria、 powder and microcapsule.(2) Using different substrates to different combinations of mangrove plant pot seedling inoculation test. The results show that the ZH5, ZH15is obvious for B. gymnorrihiza and K. candel of growth promoting effect in different substance, which in the local soil B ZH5has good performance on the B. gymnorrihiza on root weight, above weight, total weight, respectively increased48.83%,66%and48.83%, ZH15has good performance on the K. candel of in turn indexs, respectively increased104.91%,106.08%,105.23%; nitrogen and phosphorus absorption comprehensively, in the local soil+Ca3(PO4)2D the different combination bacterias on mangrove seedlings was evidently influence on the amount of total N, P, and the strains with good effect of ZH5> ZH15> ZH14. And vaccination is advantage to reduce the soil pH, salinity, overall ZH5and ZH15is suitable for different substrate, they can be considerd to applicate in the process of promoting.(3) Combination with fungicide and fertilizer on mangrove plants,the results show that the influence of Combination with ZH5, ZH15and fertilizer on mangrove growth is the best model. Add fertilizer T1in the soil, the combination strains on the B. gymnorrihiza and K. candel seedings has the important promoting role, including inoculate ZH5has good performance on the K. candel seedings on stem height, stem diameter, above N, root N, above P, respectively increased44.50%,46.43%,16.74%,27.91%,16.74%; ZH15has good performance on the B. gymnorrihiza on root weight, above weight, total weight, respectively increased46.35%,40.86%and42.75%. In the soiladd fertilizer T2, the K. candel seedings all death in the process of test, the combination of bacteria influence on the B. gymnorrihiza seedlings each has differences, including ZH5has good performance on the B. gymnorrihiza on stem height, stem diameter, root weight, above weight, total weight,respectively increased27.81%,5.78%,89.46%,96.25%,89.46%, and combination with fungicide and fertilizer is helpful to supply nutrients required for plant growth in the soil.(4) Through potted experiment, compare to effect of different PGPB dosage forms inoculated on B. gymnorrihiza seedlings, the results show that:the effect of different dosage forms of grows has difference after inoculation, and the mixture of strains of the same dosage forms is better than that single dosage forms, the promote order was strains liquid> microcapsule> strains powder,the best fungicide for ZH15liquid, ZH15glue, ZH5glue. The ZH5liquid has good influence on the stem height,stem diameter respectively increased34.14%,18.854%; ZH15liquid has good influence on stem height, above N, root P,above P respectively increased36.60%,51.36%,25.13%,51.36%, ZH15glue has good influence on the stem diameter, root N and P respectively increased19.557%,22.21%,17.03%, and the soil nutrient supplement the needs of plant growth, soil salt bacteria and bacteria powder after falling, suggests that bacteria agent to a certain extent, can improve soil salinity conditions.(5) Comparing with promoting effective of different PGPB inoculation with two dosage forms of strains liquid and strains powder on Casuarina seedlings,the results show that:the strains liquid and strains powder can obviously increase the growth of Casuarina seedlings,including ZH15fluid, ZH15powder has good influence on height, diameter respectively increased56.13%and42.12%,56.13%and56.13%. ZH5liquid, ZH15liquid has good influence on the root weight, above weight respectively increased275.29%and191.88%,147.55%and275.29%.Effect of the inoculation treatment on above of the total N content is greater than root of the total N content,and the effect of strains poeder on the total P content is superior to the strains liquid inoculation,the effect of PGPB inoculants is evident on the soil,by increasing the available nutrients of N、K on the soil,and reducing the pH,so it is significative to improve the coastal barren sand.