Piatycodon Grandiflorus Anther Development Period And The Resarch Of Tissue Culture

This research use platycodon grandiflorum anther from7different kinds of germplasm as the explants, there are "Korea jiangyuan"、"Jilin specialty"、"Longjing local"、"Neimeng platycodon"、"Taijie No.one"、"Sancheng purple-flower" and "Changan white-flower".We use conventional slice-pressing method to study the differencese of microspore development from leader branch buds of different germplasm and the characteristic between the leader branch and second branch buds of the same germplasm.Use MS and N6as the basic culture,6-BA and KT as the cell division element, NAA、IAA and2,4-D as the auxin, made12kinds of callus inducing medium of different hormone types and concentrations, N6+1.0mg/L6-BA+0.5mg/L NAA as the differential medium, to study the influence of the nduction rate and differentiation rate of platycodon grandiflorum anther callus, in which inducing medium use different kinds of basic culture and different hormone types.Use MS+1.0mg/L6-BA+0.5mg/LNAA as the callus inducing medium. In differential, use N6as the basic culture, GA-3and6-BA as the cell division element, NAA、IAA and2,4-D as the auxin, made6kinds of differential medium of different hormone types and concentrations, to be aimed at to choose the better differential medium of platycodon grandiflorum anther tissue culture. Use the anther of two-year "Sancheng purple-flower" germplasm, which the microspore development was in mid-late uninudeate as the expants to study the influence of anther tissue culture by different low temperature and time pretreatment and different inoculation methods treatment.The results are:1、There are differences between the leader branch buds pollen development period of different germplasm, and in there microspore development "Korea jiangyuan" leader branch bud is the longgest;"Jilin specialty"、"Sancheng purple-flower"、"Changan white-flower" and "Longjing local" are small.2、There are also differences in pollen development period between the leader branch and the second branch from the same germplasm, shows that:the leader branch buds is earlier than the second branch.3、MS as the basic culture in inducing medium, the effect is better than N6; The highest callus inductivity of inducing medium is MS+1.0mg/L6-BA+0.2mg/L2,4-D; AS the cell division element,6-BA is better than KT both in induction rate and differentiation rate;2,4-D as the auxin, the induction rate and differentiation rate of callus are both better than NAA, IAA is the worst. And use both KT and IAA in inducing medium, it is hard to induce the callus, even though induce to grow up the callus, but the quality of the callus is poor, and the colore is deep yellow and grow slow.4、The differentiation rate of N6+1.0mg/L6-BA+0.5mg/LNAA differential medium is the highest;6-BA as the cell division element, the differentiation rate is obviously better than GA-3; NAA as the auxin, the differentiation rate are both better than2,4-D and IAA.5、As the time of the low temperature pretreatment growth, the callus average inductivity of platycodon grandiflorum anther tissue culture rise, but the differentiation rate drop. The anther in4℃temperature pretreatment, the callus average inductivity and differentiation rate of platycodongrandiflorum anther tissue culture are both the highest.6、In6kinds of anther inoculation methods, the better induction efect and differentiation effect are take out the both ends and cut in the longitudinal and the transverse of1/2.