| This research mainly focuses on the bait matter of breeding process ofApostichopus japonicus. The solid-state fermentation was used in this research. Andthe raw material was composed of different organic matters and mixed bacteria.Kelp residue and brewer’s grain were selected as the main raw material. Ultimately,not only the utilization of fermented food can be improved, but also the pollution tothe environment can be reduced. We hope to provide a theoretical basis for theindustrial production of fermented bait.In this study, kelp residue and brewer’s grain were selected as the main rawmaterials. Shell powder, algae and other substances were selected as the accessories.The ratio of kelp residue and brewer’s grain was1:0,0:1and1:1. Every12h,moisture content and pH in the fermentation process in the system were measured.Different levels of fermented materials’ temperature was detected every morningand afternoon. To make normal fermentation, we should add buffer substances, turnthe fermentation system, etc. We should also preliminary assess the fermentationsystem by sensory indicators.Under normal conditions of fermentation, the fermentation product of threemain nutrition indicators and enzyme activity were measured. The results were asfollows: when the ratio of kelp residue and brewer’s grain was1:1, the crude proteincontent of fermentation product increased from17.09%to23.45%. Under optimumfermentation conditions, the crude protein content was up to27.44%. The crudefiber reduced from13.97%to7.81%. In addition, the content of soluble sugar andTCA-NSI increased to some content. The study of enzymes included cellulase,neutral proteases and acid proteases. Similarly, when the mixture of kelp residueand brewer’s grains were selected as the main raw material, the cellulose activitywas consistent with the degradation of crude fiber. The maximum neutral proteaseactivity was108.98U/g which was consistent with the content of TCA-NSI.Finally, the feeding experiment of Apostichopus japonicus was conducted. Interms of growth performance, except the fermentation group which regard the kelpresidue as the main raw material, the other two fermentation groups were better thanthe control group. When we fed the product which was fermented by the mixture of kelp and brewer’s grain, the daily weight gain (ADG) reached about0.57g, dailyfeed intake capacity was about3.93g. To the fermentation groups, the number ofmicroorganisms in sea cucumber gut was higher. The fermentation group whosemain raw material was brewer’s grain had a maximum value with1.46×107cfu/mL.The number of microorganisms in aquaculture water which fed fermentationproducts was higher, the maximum number was1.63×107cfu/mL. The lower CODvalue and the concentration of nitrite indicated that the growth environment ofApostichopus japonicus is better than the control group. |
