Research On Labeling Several Kinds Of Fungi Polysaccharides By Using Rhodamine B

In this study, Rhodamine B was used as a fluorescent material to label a number of fungipolysaccharides including Ganoderma applanatum, Polystictus versicolar, Auriculariaaurricula, Tremella fuciformis, Gloeostereum incarnatum, Cordyceps sp, and Hericiumcaput-medusae, based on a theory that coupling reaction can occur between the reducing end offungi polysaccharide and the active amino group. Results obtained are summarized as follows:(1) Fungi polysaccharides were labeled by using Rhodamine B as a fluorescent material.The result shows that the labeling efficiency of Ganoderma applanatum is better than that ofother selected fungi polysaccharides.(2) We choose the fluorescent labeling of Ganoderma applanatum for further study bycomparing the efficiency of combination of Rhodamine B and several fungi polysaccharides.(3) Single factor experiment was carried out on Ganoderma applanatum labeled byRhodamine B. The effects of reaction interval, pH, and temperature on Rhodamine B labelingefficiency were studied.(4) Based on the results of single factor experiment,three reaction factors described abovewere optimized by using response surface method. Optimal conditions obtained for employingRhodamine B to label Ganoderma applanatum are: reaction interval of23.10h, pH of5.23, andtemperature of25.83℃. We estimated the maximum OD value of sample after being labeledwas0.135at a wavelength of552nm with the analysis of Design-Expert8.05b software.(5) The effect of Ganoderma applanatum before and after fluorescent labeling on theinhibition rate of cell SWWC1116was studied by using MTT colorimetry. The results show thatthe fluorescent labeling has rare effect on the inhibitory effect of Ganoderma applanatum forcell SWWC1116.