| Amphiprion ocellaris, Perciformes, Pomacentridae, Amphiprion. It is a kind ofimportant marine ornamental fish. Which itself has a beautiful appearance and easy tofeed. It is the preferred species of ornamental fish enthusiasts and Mainly distributedin Southeast Asia and the Western. Pacific. In this study, the main digestive enzymesin Amphiprion ocellaris were fully discussed, included protease, amylase and lipase.Analyzed of environmental factors such as pH, temperature and salinity effects ondigestive enzyme activities of Amphiprion ocellaris. The connection between itsfeeding habits and digestive enzymes was researched.(1)The protein digestion of Amphiprion ocellaris were mainly in the stomach andintestines, and foregut had Strong ability to digest. Starch digestion by the intestinesand liver, And digestion of fat from stomach and intestines.(2)The digestive enzymes of juvenile was studied. The optimum pH, teperatureand salinity of stomach, liver, foregut, midgut, hindgut are pH2.240℃30‰, pH7.540℃25‰, pH7.040℃25‰, pH7.040℃25‰, pH8.040℃25‰, and for theamylase are pH7.040℃30‰, pH6.035℃25‰, pH7.030℃25‰, pH7.030℃25‰,pH7.030℃25‰, for lipase are pH7.540℃25‰, pH7.040℃30‰, pH7.540℃25‰, pH7.540℃25‰, pH7.540℃25‰. And the digestive enzymes of adult wasstudied. The optimum pH teperature and salinity of stomach, liver, foregut, midgut,hindgut are pH2.640℃30‰, pH8.040℃30‰, pH7.540℃25‰, pH7.540℃25‰,pH7.540℃25‰, and for the amylase are pH7.040℃25‰, pH6.035℃25‰, pH7.530℃25‰, pH7.530℃25‰, pH7.530℃25‰, for lipase are pH7.540℃25‰,pH7.040℃30‰, pH7.540℃25‰, pH7.540℃25‰, pH7.540℃25‰.(3)In its process of growth, each part of its digestive tract and the liver’s ability tosecrete digestive enzymes and digestive function gradually improved and perfected.The juvenile protease activities was higher than adult. Protease activity wassignificantly higher amylase and lipase. As a reference, for Amphiprion ocellarisartificial bait composition ratio and the feeding of judgment. |
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