Studay On Tissue Culture Of Liquidambar Styraciflua

Liquidambar styraciflua, an exotic tall deciduous tree species introduced from theUSA, belonging to the family of Hamamelidaceae, Because of its strong adaptability,fast growth, bright colors of autumn leaves, it is considered one of the most excellentspecies in landscaping. In this experiment, Liquidambar styraciflua seeds weregerminated to obtained, then the cotyledon and hypocotyl were used as explant for theinduction of callus formation and differentiation.Stem tips were used for multiplication.Suitable culture medium were developed for each stage of the process of tissue culture,producing young plants of Liquidambar styraciflua successfully. This established astable regeneration system for Liquidambar styraciflua through tissue culture. Themain results are as follows：(1) Sterilization of Liquidambar styraciflua explants. Experiments with seedsas explants, the appropriate sterilization processing is:75%alcohol30s,20%NaClOprocessing25min, the germination rate was64%.(2) Liquidambar styraciflua cotyledon and hypocotyl callus induction anddifferentiation. On cotyledon callus induction and differentiation, is the best mediumformula:1/2MS+6-BA2.0mg/L+ZT0.2mg/L+NAA0.1mg/L, Induction rate was100%,the differentiation rate of71.9%. The best medium hypocotyl callus induction ofdifferentiation: WPM+6-BA0.2mg/L+ZT1.0mg/L+NAA0.1mg/L, the differentiationrate of50.65%.(3) Multiplication culture of regenerated Liquidambar styraciflua plants. It isbetter to take twice-subculture approach, the initial subculture was mainly forproliferation and its appropriate medium was WPM+6-BA1.0mg/L+NAA0.1mg/L,proliferation factor of5.4. The second subculture was mainly for getting sound tissueculture Plants and its appropriate medium was WPM+6-BA0.5mg/L+NAA0.2mg/L,The average seedling length is2.75cm. when added200mg/L HL can improve the multiplication factor and seedling height; PVP100mg/L can decrease the browningrate.(4) Rooting culture of Liquidambar styraciflua. Rooting rate, average root lengthand average number of lateral roots were used as indicators for screening culturemedium, the best medium was M22：WPM+IBA0.2mg/L+1.6%DA-60.01mg/L.