Function Research On FRI Homologous In Soybean

Soybean is a typical short day plants, but on the path of flowering regulation was not very clear. And as the model plant Arabidopsis thaliana, flowering regulation studies of relatively clear, flowering in Arabidopsis thaliana regulated network, there are four main factors that affect plant flowering approaches, namely GA way, spring approaches, cycle routes, independent way. In this regulatory network, FLC is in hub locations are negative regulators of flowering, SOC1, by inhibiting the integration of flower factor inhibits flowering in FT. In addition to the above four approaches, there is a FRI-dependent way, FRI is the key affect the flowering of Arabidopsis genes, can promote the expression of FLC delayed flowering. After homology and found no FLC homologous genes in soybean, but contains other FLC control-related genes, like FRI, FLD, FVE. These genes (FRI), what function in the soybean? If you introduce to soybean soybean gene regulation in Arabidopsis FLC will cause change? These two parts is the main part of my experiment. Also, in the process of tissue culture of soybean found rooting of tissue culture of soybean using hydroponic methods, carried out a study on the water-cultured rooting.First,study on features of a homologous gene, soybean FRI. Homologous pairs (www.soybase.org and www.ncbi.NLM.NIH.gov) analysis showed that the FLC does not exist in the soybean genome homologous genes, but with two with FRI has sequence similarity to genes, respectively named GmFRI-1and GmFRI-2,RT-PCR test results indicate that GmFRI-1and GmFRI-2are constitutive expression in soybean, indicating they may have an important function in the soybean. GmFRI-1and GmFRI-2full length CDS into Arabidopsis thaliana (Col) ectopic expression, no obvious phenotypic changes were observed. FRI high homology in CDS and transferred to the n-Terminal part of Arabidopsis thaliana, find that5GmFRI-2in homozygous transgenic plants has obvious phenotypes of early flower and pollen sterility, but GmFRI-1phenotypes of transgenic plants showed up early. Construction of RNA interference of two genes into soybeans, are unable to get the T2transgenic plants, the GmFRI-1and GmFRI-2any loss of functionality of a gene may have a deadly effect on soybean. Description of these findings, GmFRI-1and GmFRI-2in the growth and development of soybean of soybean has important functions, but its mechanism of Arabidopsis FRI-completely different features. To examine the GmFRI-1and GmFRI-2regulation of target genes, will translate its RNA interference to build soybean cell suspension, completed the transformation of suspension cell lines and transgenic expression of GmFRI-1and GmFRI-2in suspension cells decreased, but cells are able to maintain growth. The alternative system to some extent functional analysis of transgenic soybean, such as for identification, detection of target genes transcriptome changes caused loss of functionality. Application of soybean functional genomics research is expected to.Second, Arabidopsis thaliana ectopic expression of FLC in soybean. Arabidopsis gene FLC (35S promoter FLC gene CDS) through infection by soybean cotyledon section through multigenerational testing found were detected at the DNA level-positive, and you see the T5’s, strip than more positive light is detected, but the test only to find their expression is not to express. Additionally to the DNA test is positive plant, also expanded to get sent to the sequencing, gene sequencing results show that is indeed the FLC gene in Arabidopsis thaliana. The FLC gene transformation is successful, but may be modified after this gene into soybeans causes it not to express, specific analysis of the causes have yet to be studied.Third, Root-inducing is the final step for producing regenerated plantlets in the tissue culture of soybean [Glycine max (L.) Merr.]. Here an easy, efficient root-inducing system for soybean shoots is reported, which is designated as tap-water rooting system, in which tap water is (without autoclaving) used to replace the traditional agar-containing medium for root induction and growth. Our results demonstrated that the tap-water rooting system is good for shortening culture time, overcoming contamination of bacterium and fungus, increasing the rooting efficiency and decreasing the cost of tissue culture as exemplified by faster root inducing and elongating, and higher plantlet survive ratio of the tap-water system than those of agar-containing medium system. Although the amount of total roots from the tap-water system is less than that from agar plates, the plantlets from tap-water system could grow, flower and produce pods and seeds normally and no significant difference were found between the tap-water system and agar system. Hence, It is suggested that the easy rooting system is used in the tissue culture of more soybean strains..Work of these three aspects is the soybean flowering adjustment path hoping some explanation, some progress has been made, as part of the sequence into soybean GmFRI-2abortion occurs early in the Arabidopsis flower phenotype, and GmFRI-1night flower phenotype occurred, further research on the cause of these phenotypes can provide reference for the function of this gene in soybeans. FLC gene in Arabidopsis thaliana and soybeans expressions appear in silence, this thinking has also been proposed for soybean genetic transformation.