| The Ovum Mutant gene (Om) was found in the DDK mice strain. The DDK mice with the unique Om gene have a peculiarity character known as DDK syndrome. The DDK females are mostly infertile when crossed with males of other inbred strains, while DDK males exhibit normal fertility in the reciprocal crosses, as intrastrain mating. Zhao Weidong(2000)demonstrated that there exists modifier genes(Ommf)in the genetic background of C57Bl/6 mice, which lead an increase of death rate when hetero females at om locus mated with B6 Males. This research was performed to mapping the Ommf in mice chromosome4 and chromesome9 under the previous results that obtained in our Lab.The backcross population was engaged in the research to perform the mapping research。Female N2 mice (Om/+) were obtained as samples. According to the information in the MGI (Mouse Genome Informatics) database, we choose candidate SSR markers to examine their polymorphism. In this research, we use Haley-Kontt regression to analysis the QTL of live fetus (LF) in R/QTL and calculate the LOD threshold by permutation method.1. Successfully conducted the backcross population, obtain 51 samples.2. Twenty-five SSR markers in the chromosome 4 were chosen and seven markers were polymorphism. Eleven SSR markers were polymorphism when examined from the fifty candidate markers.3. The results of fine mapping experiments in mice chromosome 4 and Chromosome 9 indicate that there may have a QTL of IM in chromosome 9. The QTL of IM and LF was located in the Chromosome 4 at 66cM, which is significant. The Bayesian credible interval Analysis indicates the credible interval of QTL of LF is about 20cM, between 61.5~71cM. According to the results above, we can add more SSR marker in this interval to further the mapping experiment or we can find candidate genes in the interval from the database. Finding modifier genes that have negative functions of DDK syndrome will enhance the study of Om and do basic works for the research of embryo development pathway. |
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