Effect Of Glu On Development Block And Expression Of Relative Gene In Mouse MZT Embryo

Compared with the in vivo environment,mammals in vitro embryo-culture systems are suboptimal,as they are risk of developmental delay.The balance between ROS production and clearance is disturbed and may lead to 2-cell developmental block of mouse embryos.This study is to research developmental block from on the molecular level,investigate effects of Glu during I VC on ZGA and MZT,determine the anti-developmental block effect of Glu on mouse embryos.Expect to serve as the foundation for solving the low production rate.In this study,we investigated the effect of glutamate treated and M16 control comparing among the early,middle,late 2-cell mouse embryos,used DCHF-DA to detect ROS content,used CMF2HC detect GSH content,and analysed the expression of mRNA level of GPx gene such as GPxl and GPx4,ZGA marker gene such as Eif-1α,Muervl,Zscan4d and Hsp70.1.Results showed that:1、The 2-cell to 4-cell transition rate of 6mmol/L Glu treat was significantly higher than Ml6 control and others Glu treat;2、The ROS and GSH content among MZT stage of Glu treat had no significant difference,while ROS significantly lower than M16 contrl,GSH significantly higher than Ml6 control;3、Glu treate up regulation the expression of GPx gene of MZT embryos;4、Glu treate up regulation the expression of ZGA marker gene of MZT embryos,especially significantly increased at middle and late stage;In conclusion,the concentration of 6mmol/L Glu could maintain the dynamic balance of GSH and ROS by stimulating the activity of GPxl and GPx4 enzyme,indirectly participate in the antioxidant process of scavenging ROS;Could increase the expression of ZGA marker gene and maintain its high expression pattern of time series,directly participate in the ZGA activated process;ultimately reduce the risk of developmental block to ensure the successful completion of MZT.