Molecular Detection The Swine Hepatitis E Virus In Henan And Analyse The Comeplete Genome

Hepatitis E is a acute liver disease caused by hepatitis e virus,which is transemitted mainly by faecal-oral, has many transemitted routes.HEV has a aborad hosts. More and more evidence show that HEV is a animal reservoirs and zoonotic disease,which can be transemitted from swine to human. Swine is one of the major HEV hosts.There is a high prevalence of HEV in swines that may threaten human’s lives and the safety of food. We will have a primary research on swine HEV in Henan.133 swine serum samples collected from Zhengzhou, Luoyang, Xinxiang, Jiaozuo and Zhoukou were tested by ELISA for the presence of IgG antibodies against HEV. 200 pig fecal samples from Zhengzhou, Xinzheng,Zhongmou, Jiyuan and Puyang were tested for the presence of HEV RNA by reverse transcription nested polymerase chain reaction (RT-PCR).Results showed 79.70% of the serums were positive for antibody against HEV,and 7.5% of the fecal samples were positive for HEV RNA. Nucleotide sequence analysis showed that three HEV strains detected in Henan belongs to genotypeⅣ. Phylogeny analysis showed it had a high identity with Hubei strain.To further study the characteristic of swine HEV in Henan,we set 8 pairs of primers to get the genome of Henan HEV strain JY40 by RT-PCR which named“HN-JY40”.The sequence was 7252 bp excluding the poly(A )tail, and including 44.88% of A/T bases with 55.12% of C/G. The 5’UTR has 9 bases ,and 3’UTR has 69 bases.ORF1 comprised 5124 nt that encoded 1708 amion acids; ORF2 comprised 2025nt that encoded 675 amion acids; ORF3 comprised 345nt that encoded 115 amion acids. The result of analyse showed that HN-JY40 shared 93% identity at nucleic acid level.