Research Of Super-resolution Confocal Microscopy With Structurered Detection

Confocal microscopy lateral resolution decreases due to the finite-sized pinhole. From the perspective of spatial frequency, with structured illumination super-resolution and confocal microscopy principle combined, the spacial exploration light sensitivity is modulated as spatial modulation, so as to improve the lateral resolution of a confocal microscopy system. Based on imaging principle of confocal microscopy with finite-sized pinhole detection, spatial modulation super-resolution method is introduced intoconfocal microscopy system, confocal microscopy lateral super-resolution and research of coherent imaging and non coherent imaging principle in confocal super-resolution method based on structured detection. The main work done includes:Firstly, the coherent imaging theory of confocal microscopy system with a point detection is studied, by deducing the confocal microscopy coherent imaging formula and coherent transfer function and analyzing the influence of detector lateral offset in CTF and axial laminating ability. The partially-coherent imaging principle of confocal microscopy with finite-sized pinhole detection is studied, by deducing in coherent illumination condition partially-coherent imaging formula and the transmission cross-coefficient and analyzing the influence of the pinhole in lateral edge-setting intensity response characteristics and the axial intensity response characteristics. Non-coherent imaging principle of confocal microscope with finite-sized pinhole detection is studied, by deducing the confocal microscopy non-coherent imaging formula and optical transfer function and analyzing the effect of pinhole on optical transfer function.Secondly, non-coherent imaging principle of confocal microscopy based on structured detection is researched, by establishing confocal microscopy incoherent imaging system model based on structured dectection, deducing confocal microscopy non-coherent imaging formula and optical transfer function. The whole process based on structured detection of confocal microscopy incoherent imaging system for image reconstruction is given, with the use of different phase structured detection of phase, operating in the frequency domain and realize the separation of the spectrum of the object, so as to make the image reconstruction. And the effect of structured detection function parameters on non-coherent imaging system lateral and axial resolution of confocal microscopy based on structure detection is discussed.Thirdly, approximately-coherent imaging principle of confocal microscopy based on structured detection is researched, by establishing confocal microscopy approximately-coherent imaging system model based on structured dectection, deducing confocal microscopy approximately-coherent imaging formula and coherent transfer function. The whole process based on structured detection of confocal microscopy approximately-coherent imaging system for image reconstruction is given, with the use of different phase structured detection of phase, operating in the frequency domain and realize the separation of the spectrum of the object, so as to make the image reconstruction. And the effect of structured detection function parameters on approximately-coherent imaging system lateral and axial resolution of confocal microscopy based on structure detection is discussed.Lastly, confocal microscopy system experimental platform based on structured detection is designed and constructed. In approximately-coherent imaging experiments, the lateral resolution of confocal microscopy system based on structured detection is 1.7 times as much as that of conventional coherent microscope. In fluorescence non-coherent imaging experiments, imaging contrast significantly improves. Experimental results verify the correctness of the method.