Study On Pseudomonas Sp. ZXY-1 Synchronous Bacterial With The Function Of Synchronous Degradation Atrazine And Ammonia

With the continuous development of our economy, agricultural non-point source pollution has become the main source of water pollution in China, due to the use of a lot of chemical fertilizer and pesticide in agricultural production, thus agricultural non-point source pollution is serious year by year, bringing serious environmental risks, especially to the problem of excessive ammonia nitrogen, which has already become a serious surface water exceeded the imp ortant non-point source pollutants. The control of non-point source pollution has attracted the attention of the government and researchers. Due to its high efficiency, green, pollution-free, low investment cost and so on, it has become an effective method to control agricultural non-point source pollution in recent years. But under natural conditions, accompanied by the process of agricultural production, pesticides( atrazine was used broadly) and fertilizer pollution often exist at the same time, atrazin e and other biological nuisance pesticide due to its biological toxicity effect of biological agents that can lead to biological treatment of reducing the efficiency of agricultural non-point source pollution. So there is an urgent need to screen out toler ance to atrazine toxicity, and can also remove ammonia strains,which is used to improve the surface source pollution purification efficiency. In this paper, we successfully screened a strain that can remove atrazine and ammonia nitrogen, expounding the application of high strain method to deal with the agricultural non-point source pollution, so as to realize the synchronous degradation of pollutants in agricultural non-point source pollution.In this study the strain is from pesticide factory of Jilin Che mical Co., Ltd. contaminated sludge isolated,I named it Pseudomonas sp.ZXY-1.And the functional strain was identified in morphology, physiology and biochemistry., being domesticated, observing the farmland back water to the pesticide atrazine and ammonia nitrogen removal.In this experiment, sp. ZXY-1 strain Pseudomonas owns a function of atrazine removal, and explored the removal of atrazine in the optimal conditions, so in different temperature, different p H value, different rotate speed, on the removal of atrazine and the effect of different initial concentration of ammonia nitrogen of atrazine and other conditions, and the removal efficiency of ammonia nitrogen between strains in the absence of atrazine under the function of stra in on atrazine removal and nitrogen removal efficiency should be improved, so as to further to improve the function of ammonia removal efficiency in single strains, the various factors(different temperature, different p H value and different rotation speed and different C/N) on the basis of the design of five factors and three levels response surface test and comparison of atrazine existence condition of strain difference on the removal efficiency of ammonia nitrogen. In addition to the further improvement of the efficiency, after optimization of response surface experiments, the functional strains of ammonia nitrogen removal efficiency is significantly improved, the results of this study are as follows.The research results show that the strain Pseudomonas sp. ZXY-1 in temperature to 30 oC, p H 7.0, shaker speed is 150r/min, initial concentration of atrazine to 100mg/L-200mg/L strains of atrazine removal efficiency best and highest can be up to 99%; in pesticide- free case, when the temperature is 30 oC, p H 9.0, shaker speed is 150 r / min, C / N ratio was 15 strains Pseudomonas sp. ZXY-1 of ammonia nitrogen removal efficiency can reach the maximum, maximum degradation rate of up to 96%; in containing atrazine, in p H = 8.0, T=32 DEG C, shaker speed =150r/min. = 10, glucose as carbon source, ammonia nitrogen removal rate reached the highest at 76.25%. Through the use of efficient methods of microbial control agricultural non-point source pollution problem and realize the use of pure microbial simultaneous removal of atrazine in agricul tural non-point source pollution, ammonia and other pollutants, this study compared with other innovation research is using pure strains can realize the pesticide atrazine and ammonia nitrogen of simultaneous removal and for the use of the application of microbiological methods of agricultural non-point source pollution control and the research provides some guidance and reference.