The Design Of Novel Fluorescent Probe And Performance Research

Desoxyribonucleic acid (DNA) is an important component of all organisms, it carries the genetic information in the process of biological evolution and is the material basis of gene expression. It plays an important role in biological growth, development and reproduction. The ruthenium(Ⅱ) polypyridine complexes have properties of large Stokes shift, long luminescence lifetime, and high photochemical stability. Complexes with polypyridyl ligands, because of a combination of rigid chiral structures spanning all three spatial dimensions which can adapt the DNA helix have prominent DNA binding properties. So the study of the interaction of ruthenium complexes with DNA has important significance in therapy drugs or structure probe.Two ligands 2-[4-(9H-carbazol-9-yl)phenyl]-1H-imidazol[4,5-f][1,10]phenanthroline, 2-[4-(9H-imidazol-9-yl)phenyl]-1H-imidazol[4,5-fJ[1,10]phenanthroline and their ruthenium complexes [Ru(bpy)2(CpipH)](PF6)2, [Ru(bpy)2(IpipH)](PF6)2 have been synthesized and characterized by infrared, HNMR, ultraviolet fluorescence. The interaction of ruthenium complexes with CT-DNA was measured through spectrocopy method, viscosity test. Primary work includes the following aspects:1.This paper introduces DNA and ruthenium pyridine metal complexes, the research progress of metal complexes and DNA interaction, role model, research methods and research significance.2. Two ligands 2-[4-(9H-carbazol-9-yl)phenyl]-1H-imidazol[4,5-f][1,10]phenanthroline, 2-[4-(9H-imidazol-9-yl)phenyl]-1H-imidazol[4,5-f][1,10]phenanthroline and their ruthenium complexes [Ru(bpy)2(CpipH)](PF6)2, [Ru(bpy)2(IpipH)](PF6)2 are synthesized. Through the means such as infrared, HNMR. ultraviolet and fluorescence, the complexes were characterized and analyzed. At the same time, through spectroscopy research, electrochemical test and theoretical calculation further discusses the nature of the two complexes.3. The DNA binding behaviors of complexes were studied by using spectroscopic method viscosity measure. Results suggested the complex binds to DNA via an intercalative mode and complex [Ru(bpy)2(CpipH)](PF6)2 has stronger binding force than complex [Ru(bpy)2 (IpipH)](PF6)2.