Synthesis And Recognition Properties Of Fluorescent Small Molecules Containing Reactive Functional Groups

In the past ten years, biological chemical industry of fluorescence sensors are used more and more widely, developed a series for human detection in harmful substances of organic molecules regardless of biology, medicine and people’s life plays a vital role. Because of it’s high sensitivity, high selectivity, simple operation, low detection limit, and biological imaging in living cells, the fluorescence chemical sensor has been favored by researchers.Coumarin dyes have strong optical properties, with the 3,4 - two methoxy thiophenol synthetise of thiol amino acids connected with chemical sensor based on diethylamino coumarin (Probe 1), the emission spectrum of selective Probe 1 on thiol amino acid interference and response time were studied by ultraviolet spectroscopy and fluorescence, the test results show that the Probe 1 547nm in the fluorescence emission spectra of GSH with high selectivity, at 475nm on Cys, Hcy also performance a good selectivity, in order to distinguish between Cys and Hcy, will change the pH of the buffer solution was 6, the emission wavelength of 445nm possess good selectivity on the Cys, and can distinguish between Cys and Hcy. The response time of Probe 1 and GSH can reach the balance in 90s, because of its fast response time, it can greatly improve the efficiency of detection of thiol amino acids, which makes the detection process more convenient. We have successfully achieved the fluorescence imaging experiments of thiol amino acids in living cells, and the Probe 1 and GSH, Cys, Hcy are 1:1 stoichiometric reaction. Therefore Probe 1 performance the prospect of qualitative and quantitative determination of thiol amino acids in environmental systems and biological systems.The DPP as a with high Stokes shift of the fluorescence dye which has potential as a near infrared fluorescent sensor. Therefore, the and benzothiazole sub ammonium salt is formed by the connection of the probe 2. And by using ultraviolet absorption spectra and fluorescence emission spectroscopy to probe the physical properties and the Cation Anion Sensing and identification were studied. Test results showed that the Probe 2 in acetonitrile and Tris buffer solution had no fluorescence emission at the time of 539nm excitation. When CN- was added in the wavelength of 596nm fluorescence intensity of fluorescence peaks appeared to increase to about 35 times, so as to achieve the recognition of CN’. The probe has a high selectivity for CN-and CN" stoichiometric ratio is 1:1.The Probe 2 has application potential for detecting trace CN-in environmental system and biological system.