Study Of New Raman Enhanced Substrate For Rapid Detection Of Paes Plasticizers

Objective: Phthalates plasticizers（PAEs） are global environmental contaminants, which present in a wide variety of products. Human exposure to PAEs through various ways, and the long-term accumulation cause the nervous, reproductive and developmental toxicity. The conventional detection methods of PAEs include gas chromatography-mass spectrometry（GC-MS）, high-performance liquid chromatography（HPLC）, fluorescence spectrophotometry and so on. However, complex sample preparation, time-consuming, high cost, less efficiency, and other factors limited their application. Surface-enhanced Raman scattering（SERS） technology can provide detailed information on chemical constitution and molecular structures. Furthermore, it offers significant advantages, such as fast, accurate, high sensitivity, and it has been widely used in environmental detection, materials science, biology, medicine, and other fields. In this study, uniform, stable and reproducible Ag nanofilm Raman enhancement substrate were prepared by spraying method to realize the rapid detection of PAEs.Methods: First of all, we synthesized uniform, good soluble, and stable Ag nanoparticles via microwave-assisted method, using amino acid as reducing agent, soluble starch as surfactant and stabilizer. Secondly, the Ag nanoparticles were assembled on the clean glass substrate using spraying method to obtain the Ag nanofilm Raman active substrate. Again, analysis the conventional Raman spectra to get their common and unique characteristic absorption peak, such as butyl benzyl phthalate（BBP）, di-butyl phthalate（DBP）, di-iso-nonyl phthalate（DINP）, di-n-octyl phthalate（DNOP）, di-（2-ethyl hexyl） phthalate（DEHP）, di-iso-decyl Phthalate（DIDP）, which were most attended, and the simplest structure like di-ethyl phthalate（DEP）, di-methyl phthalate（DMP）. Finally, the Ag nanofilm enhanced substrates were applied to the rapid detection of phthalates plasticizers, such as detect the BBP in blood,identify the mixture of BBP and DEP in blood, and distinguish between DEP and DMP in blood.Results:Ag nanoparticles via microwave synthesis show uniform, with an average size of about 40 nm, stabilization at room temperature for several months, morphology and properties of almost not changed. The Ag nanoparticles synthesized by one time can be used for spraying assembly more than 600 times, and deposited uniformly and orderly on a glass substrate. The common characteristic peaks of PAEs at 403, 650, 1044, 1128, 1167, 1581, 1601, 1728 cm^-1 obtained by conventional Raman spectra. On the basis, prominent features peaks at 351 cm^-1, 1495 cm^-1 of DEP molecular, 1285 cm^-1 1044 cm^-1 of BBP molecular, and 971 cm^-1 of DMP molecular can be identified through the analysis of SERS spectra.Conclusions: The Ag nanoparticles prepared by microwave-assisted method display more advantages, like controllable morphology, uniform size, stable performance, long-term preservation. They can be synthesized in large quantities, and the simple preparation process, short time-consuming, and equipment, reagents, materials prices cheap, low cost. Spraying assembly technology for the preparing of Ag nanofilm highly ordered, activity and high sensitivity, can detect and identify the mixed plasticizer in blood samples with strong interference, and it has widely application prospect.