Reactive Fluorescent Probe For The Detection Of Hydrogen Peroxide And Glucose

Fluorescent sensors have the advantages of high sensitivity and high selectivity. Due to their low cost and easy operation, they are widely used in biology, chemistry, medicine and other fields. In this research, a new method based on a reactive water-soluble fluorescent probe was developed and utilized for quantitative detection of hydrogen peroxide and glucose.A new method of highly sensitive fluorescent enhancement for detection of hydrogen peroxide and glucose in the samples of food and biology was developed. In the presence of ferric iron ion and hydrogen peroxide, m-aminobenzene sulfonic acid as monomer was oxidized into a water-soluble conjugated polymer, and which was used as fluorescent indicator probe.The effect of monomers and metal ions on the fluorescent intensity was investigated in detail. The results indicated that fluorescent intensity of the reactive system enhanced with the increase of the amount of hydrogen peroxide in the presence of ferric iron ion and m-aminobenzene sulfonic acid. So the present the reactive system was utilized for the detection of hydrogen peroxide. The effect of concentrations of monomers, metal ions, kinds of buffer solutions, pH values, and the reactive time were discussed. The reaction mechanism of the fluorescent probe was studied by UV-Vis spectroscopy and’H NMR. Under the optimal experimental conditions, the standard curve of hydrogen peroxide were obtained. The fluorescent intensity had good linear relationship with the concentration of hydrogen peroxide. The linear range were 9×10-7mol/L-1 ×10-5mol/L and 1×10-5mol/L-1×10-4mol/L. The correlation coefficients were all above 0.99. The detection limit was 5×10-7mol/L. The method was used to detect the amount of hydrogen peroxide in the beef tripe.Glucose can be oxidized by glucose oxidase, and release hydrogen peroxide. Thus, the mentioned method can also be used to detect glucose.The fluorescent intensity of the system increased with the increase concentration of glucose. The effect of concentration of the glucose oxidase and the reactive time on the fluorescence intensity was investigated. Under the optimal experimental conditions, the standard curve of glucose was obtained. The fluorescent intensity showed good linear relationship with the concentration of glucose. The linear range was 1 x 1 ×10-7mol/L-5× 10-6mol/L and 1 × 10-5mol/L-5 × 10-5mol/L. The correlation coefficients were above 0.99 and 0.98, respectively. The detection limit was 1×10-8mol/L. Finally, the concentration of the glucose in the human serum was detected by the present method.