The Study Of Ganoderma Lucidum And Resistant Starch’s Coordinated Intervention On Glucose And Lipid Metabolism Disorders

Ganoderma is a fungus that belongs to the drug, as well as food, and the seed of ganoderma, ganoderma lucidum (GL), is composed of ganoderma lucidumpolysaccharides, triterpene, nucleoside, sterol, alkaloid, furan derivatives, amino acids, peptides, fatty acid, mineral elements and so on. GL, has many pharmacological effects. Resistant starch (RS) is a kind of starch which cannot be digested and absorbed in healthy human’s small intestine but can directly reach to the large intestine to play a role. Studies have shown that RS has some functions, for example, hypoglycemic, hypolipidemic, weight control, improving intestinal health, promoting digestion and absorption of minerals, and increasing nutrition, is widely used in various fields such as medicine, food, chemical industry and so on.In this experiment, type 2 diabetic rat models was established by injecting streptozocin (STZ), ganoderma lucidum was embedded by resistant starch to study the synergetic intervention effects on the oxidative stress and the related gene transcription levels involved in glycometabolism and lipid metabolism. On this account, the mechanisms of the effects of synergetic intervention on oxidative stress, glycometabolism and lipid metabolism were studied.This experiment mainly includes three parts. That is, firstly, using resistant starch and ganoderma lucidum as raw materials, the embedding product was prepared, scanning electron microscopy morphology (SEM) was employed-to observe the sample’s morphology and it’s digestive characteristics.Secondly, to investigate the mechanism of RS and GL on glycolipid metabolism and oxidative stress in type 2 diabetic rats, diabetic rat models was established by injecting STZ, and the effects of RS and GL on blood glucose, blood lipid and oxidative stress index were investigated. Results show that both RS and GL feeding only can reduce the blood glucose and lighten glucose oxidative stress damage caused by anti-diabetic to a certain extent. RS exhibited better effect than GL in this respect. While GL has an advantage in improving lipid metabolism disorder than RS. By this experiment we can find that the synergetic intervention of RS and GL can futher improve the blood glucose level, meanwhile the lipid metabolism and anti-oxidation ablility also had been effectively improved. The antioxidant ability was further enhanced through the synergistic intervention with RS. Thus, the mode of synergistic intervention and simultaneous consumption of RS and GL can effectively reduce blood glucose, improve lipid metabolism, and enhance the ability of antioxidative stress. This mode is an efffective dietary intervention method for preventing the diabetes and improving symptoms of diabetes.Finally, to study the relevant mechanism, real-time PCR was conducted to analyse the related genes in experimental diabetic rats about the liver glycometabolism and lipid metabolism. Results showed that, compared with normal group and diabetes blank group, the embedding group can significantly improve the mRNA’s relative expression level which was related to relative genes involved in glycometabolism and lipid metabolism. At present this is the first research of using RS and GL as the raw materials to study the internal mechanism of glucose and lipid metabolism disorders.