Studies On The Extraction And Microencapsulation Of Blackberry Seed Oil

The blackberry seed, the processing by-product of blackberry, is often discarded as waste, not used efficiently, and even causing environmental pollution. Blackberry seed contains abundant nutritional ingredients. Therefore, the development and utilization of blackberry seed will has great social and economic benefits.The blackberry seed oil was extracted by supercritical CO2 fluid from blackberry seed which was vacuum freeze dried and freeze grinded. The comparision of the oil characteristics extracted with supercritical CO2 fluid under optimal conditions with soxhlet methods was carried out. At same time, blackberry seed oil micro-encapsulation with method of spray-drying process was carried out, the aim of these researches is to provide a theoretical basis for industrialized production of blackberry seed oil microcapsules and add the value of blackberry. The results were summarized as follows:The blackberry seed oil was extracted by soxhlet method from blackberry seed, and the effects of different solvents, grinding degree, extraction temperature, extraction time, solid-liquid ratio on the oil yield were investigated. The optimal conditions were determined by orthogonal test as follows:benzine as solvents, grinding degree pass 80 mu, extraction temperature 90 ℃, extraction time 2h, solid-liquid ratio 1:15(g:mL). Under the optimal conditions, the yield of blackberry seed oil was 22.86%.The blackberry seed oil was extracted by supercritical CO2 fluid from blackberry seed which was vacuum freeze dried and freeze grinded, and the effects of extraction pressure, extraction temperature, CO2 flow rate, extraction time, separation pressure, separation temperature on the oil yield were investigated. The optimal conditions were determined by Box-Behnken design as follows:grinding degree 60 mu, seed moisture content 3.43%-5.35%, extraction pressure 30 MPa, extraction temperature 40℃, CO2 flow rate 25 L/h, extraction time 60 min, separation pressure 7 MPa, separation temperature 35℃. Under the optimal conditions, the yield of blackberry seed oil was 18.60%.The characteristics of the blackberry seed oil extracted by supercritical CO2 fluid from blackberry seed which was vacuum freeze dried and freeze grinded were:yellow, clear and transparent, refraction index (20℃) 1.4792±4E-05, acid value (3.11±0.69) mg KOH/g, saponification value (181.17±2.98) mg/g, peroxide value (10.72±0.57) meq/kg, including 22 kinds of fatty acids, the content of unsaturated fatty acid was 82.31%, in which, linoleic acid 65.95%, linolenic acid 14.97%. The blackberry seed oil extracted by different pretreatment methods or/and oil extraction methods were compared in the physical and chemical index and the nourishment composition. The results showed that the quality of the blackberry seed oil extracted by supercritical CO2 fluid from blackberry seed which was vacuum freeze dried and freeze grinded was better than others.The micro-encapsulation of blackberry seed oil extracted by supercritical CO2 was carried out by spray-drying method. The result indicated that the optimal conditions from orthogonal experimental design were as follows, denaturated starch (HI-CAP 100) to gum arabic to maltodextrin was 2:1:1 (W/W), the ratio of core to wall was 0.4, TSS was 40% (W/V). The optimal spray-drying technological parameters from orthogonal experimental design were as follows, temperatures of entering wind and out wind were 180℃ and 90℃ respectively, feed rate was 20 mL/min. The micro-encapsulation efficiency of the production was 98.10%.The characters of microcapsuled blackberry seeds oil prepared by spray drying technique were evaluated. The preparation of Amur grape oil showed milkiness in colour. It provided with certain dispersion and admixture. The density, the content of moisture and solubility were about 0.81±0.07 g/cm3,2.68±0.45%,94.17±0.67%, respectively. It could meet the criteria for General micro-capsules. Through experiment, the preserved stability of microcapsuled blackberry seeds oil excelled other production. The storage lifes of two kind of production were 150 days and 26 days, respectively. Microencapsulation technology could effectively extend the shelf-life of black raspberry seed oil and enhance the storage stability of blackberry seed oil.