In this experiment, we take Cabernet Sauvignon grape seeds which are provided by Xinjiang Changyu Grape Wine Limited Company as raw materials, and detailedly discuss extraction technological parameter, decoloration technical condition and drying process condition of Cabernet Sauvignon grape seeds, and expect to provide significant theoretical basis for further processing and comprehensive utilization of Cabernet Sauvignon grape seeds. Experimental content and main results as follow:Utilize the combination of single factor experiment and response surface method to optimize extraction technological parameter of Cabernet Sauvignon grape seeds’ protein. The experiment uses lye dissolution and salt dissolution to discuss the extraction process of Cabernet Sauvignon grape seeds’ protein and shows when extracting Cabernet Sauvignon grape seeds’ protein, the method of lye dissolution has an advantage over salt dissolution. Though optimizing experiment condition, when the extracting solution’s PH reaches 11.5, ratio of liquor to material is 25:1, material grinding degree is 60 meshes, extraction temperature is 37 ℃ and the extraction time reaches 60 minutes, the extraction ratio of Cabernet Sauvignon grape seeds’ protein can reach the highest that is 53.04%.This experiment makes use of activated carbon, macroporous resin and hydrogen peroxide to decolor extractive grape seeds’ protein. Its result shows: ①When the additive amount of activated carbon is 25%, decolorization ratio reaches the maximize of 55.43%, but the loss ratio of protein reaches 40%;② The decolorization effect of macroporous resin X-5 is much better, and its decolorization ratio reaches 53.91%, but the loss ratio of protein reaches up to 23.85%③ The optimum decoloring technological parameter of hydrogen peroxide is: additive amount of hydrogen peroxide is 4%; decolorization temperature is 25℃; decolorization time is 60 minutes. Through comparing the three decolorization methods, hydrogen peroxide has the best decolorization effect, so it is optimal decolorizing agent.In order to get powdered grape seeds’ protein, the experiment adopts the spray-drying process to dry the extracting solution of grape seeds’ protein. Use single factor and the combination of uniform design experimentation to optimize sprat-drying process condition. Its result shows that under those conditions where inlet air flow is set as 0.65 m3/min; the inlet air temperature is set as 180 ℃; the feedstock flow is set as 400 m L/h and atomizing pressure is set as 200 kPa, all indexes can reach the better effect. Its collection retention is 45.43%; PDI is 73.1% and the water content is 1.03%. |