Electrochemical Studies Of Anticancer Medicines

With the development of social economy,the deterioration of the human living environment,unhealthy diet, bad habits of life and other factors will increase the risk of cancer, will directly endanger the safety of human life.lt is considered to be one of the important reasons for the global human death Treatment of cancer methods including of chemotherapy,radiotherapyoperation and traditional of TCM. anticancer drugs will be used in the process for treatment.And anti-cancer drugs by directly or indirectly interfere with the body cells of deoxyribonucleic acid (DNA), to achieve the purpose of reduce or killing human cancer cell proliferation, but if the dose is larger, can kill normal cells of live^ kidney,affect the health of the people will accompany by side effects such as diarrhea, pain at the same time. In recent years, the research analysis methods for determination of the content of anticancer drugs and drug residues in the body, has always been the focus of the researchers.Methods to study the anticancer drugs has been reported with high performance liquid chromatography,capillary electrophoresis, gas chromatography and ultraviolet spectrophotometry method.But most of these instrument are expensive, the operation steps are complicated, is not conducive to practical application. Electrochemical methods because of its simple operation, electrochemical response speed is quick, sensitive electrochemical detection and become a new research hotspot.This thesis mainly studied the electrochemical behavior of anti-cancer drugs, established a series of rapid, sensitive and quantitative methods.The main contents are as follows:1. With nano gold (AuNPs)/polyvinylpyrrolidone(PVP) as modifying agent, was prepared AuNPs/PVP/Au/CME, using Using cyclic voltammetry in pH 7.19 Na2B4O7 KH2PO4 buffer solution was researched explored in detail to the electrochemical behavior of VDS, find out the optimum measuring conditions. The results showed that the modified electrode in Na2B4O7-KH2PO4 buffer solution (pH= 7.19) by the cyclic voltammograms scanning, at 0.39V, produce a sensitive reduction peaks P1 and a less sensitive oxidation peak P2.When added with a certain concentration of VDS, P1 peak current decreased obviously, and P2 peak current changed little, and the value of the peak current decline in VDS to sheen and addition concentration in 10-8～1.0 × 1.0 × 10-4 mol/l, showed a good linear relationship, within a linear equation for the ip (uA)=5.303 v1/2-10.013588, R2= 0.99904, the detection limit of 3.2 × 109 mol/L.The AuNPs/PVP/Au/CME exhibited high sensitivity and selectivity to determination VDS.2. The electrochemical behaviors of act-D at a polymer O-Phenyenediamine and AuNPs-CS modified Au electrode (MIP-POPD/AuNPs-CS/Au/CME) were studied.A simple and efficient molecular imprinting electrochemical sensors (MIP-POPD/AuNPs-CS/Au/CME) was prepared by electro-polymerization of O-phenyenediamine in the presence of act-D based on a AuNPs-CS modified Au electrode(AuNPs-CS/Au/CME) for the selective recognition of act-D.The experiment results showed excellent selectivity and high sensitivity.The MIP-POPD/AuNPs-CS/Au/CME indicate a good linear relationship between the peak currents of act-D and its concentration in the range of 1.0×105～1.0×108mol/L with the limit of detection (S/N=3)of 2.0×105 mol/L.RSDs were 0.8～1.7%.Recoveries were 97.5～108%.The results showed that MIP-POPD/AuNPs-CS/Au/CME could accelerate the electrochemical oxidation of act-D,and could be used for the determination of act-D.3. A novel molecular imprintde polymers based on ionic liquid functionalized AuNPs-CS was prepared by electro-polymerization,which was applied as a molecular recgnition element to modified Au electrode to construct an electrochemical sensor (MIP-Ppy/IL/AuNPs-CS/Au/CME) for sensitive detection of 5-Fu.The fabricated MIP-Ppy/IL/AuNPs-CS/Au/CME was applied to electrochemical detection of 5-Fu with a wide linear range from 1.0×107～1.0×103mol/L, Al (μA)=-4.66381v+41.45062.R2=0.99092,and a detection limit of 3.0×108mol/L.The results demonstrated that the prepared molecular imprinting sensor possessed excellent selectivity sensitive and repetitive, which could be uesd for the trace detection of 5-Fu in clinical applications.