The Purification Of α-linolenic Acid In Perilla Frutescens Oil And Preparation Of Its Health Duck Sauce

Perilla frutescens, as a kind of edible and medical plant, our country has a long history of planting Perilla. It contains a lot of kinds of polyunsaturated fatty acids, in which the amount of α- linolenic acid is highest. Increasing intake of α-linolenic acid properly could keep healthy, prevent and cure atherosclerosis, reduce blood fat and so on. The byproducts, perilla frutescens dregs are rich in protein, dietary fiber, and some other nutritional ingredients. Its low comprehensive utilization efficiency caused huge waste of the resources. In recent years, attention has been drawn to perilla frutescens, which is a new type of health care resource. This paper aims at extracting perilla frutescens oil from the seeds and measuring physicochemical properties of this oil. Furthermore, α-linolenic acid was clathrated and purified. A type of health care product was made by perilla frutescens oil and dregs. The function of this product was evaluated in order to discuss comprehensive development and utilization of perilla frutescens. The results showed that:Perilla frutescens oil was extracted by hydraulic compression. The physicochemical indexes are as follows: the iodine value is 193.2 g/100 g, the acid value is 4.45.mg/g, the peroxide value is 2.23 meq/kg, the saponification value is 191.7 mg/g, the water is 0.11%, oil extraction rate is about 30.8%. Gas chromatography analysis showed that palmitic acid, stearic acid, oleic acid, linoleic acid and α-linolenic acid account for 6.85%, 2.16%, 17.09.%, 12.64% and 61.25%, respectively. The content of total unsaturated fatty acid is 90.32. In conclusion, perilla frutescens oil is a good oil resource with good qualities.The α-linolenic acid in perilla frutescens oil was enriched and purified by clathration by β-cyclodextrin assisted by ultrasonic. The orthogonal test was conducted according to a series of single factors such as ultrasonic power and time, clathration time, the mass ratio of alcohol+ mixed fatty acid to β –CD. The variance analysis showed that significant factors(P<0.05)were ultrasonic power and time, the mass ratio of alcohol+ mixed fatty acid to β –CD. The optimal technological conditions for clathration by β-cyclodextrin assisted by ultrasonic were as follows: ultrasonic power is 200 W, ultrasonic time is 25 min, the mass ratio of alcohol+ mixed fatty acid to β–CD is 1:9. Under the conditions, the amount of α-linolenic acid increases to 69.26%, the inclusion rate is 73.36%.The Sansui duck and perilla frutescens dregs were used as main materials, single-factor experiments were conducted to study the influences of the mass ratio of peanuts to perilla frutescens dregs, the additions of duck, soybean paste, perilla frutescens oil, carrageenan on texture properties of perilla frutescens seeds oil sauce. The optimal formula of perilla frutescens seeds duck sauce was determined according to sensory evaluation by L9(3)4 orthogonal test, which showed as follows: the mass ratio of peanuts to perilla frutescens dregs is 1:3, the additions of duck, soybean paste, perilla frutescens oil, carrageenan are 20%, 16%, 20%, 0.3%, respectively. The qualities of the products under this technological conditions are the best.The product was tested by animal function experiments. The results showed that mice increase weight obviously because of feeding by high fat fodder. Feeding by perilla frutescens seeds duck sauce could lose weight to a certain extent. Different additions of perilla frutescens seeds duck sauce have some influences on blood lipid levels. The total cholesterol, triglyceride and low density lipoprotein of the group feed by high-fat fodder increased obviously compared to control group(P<0.05), which demonstrated that the hyperlipidemia model of mice was established successfully. The different amount of perilla frutescens seeds duck sauce feeding test showed that low addition and modest addition of perilla frutescens seeds duck sauce impacted TC and TG of mice indistinctively, while high addition worked which could inhibited TC and TG obviously, which were 4.918 mmo L/L、1.506 mmo L/L, respectively. Low addition hadn’t distinct influences on LDL-C, while modest and high addition could enhance LDL-C. The LDL-C amount of modest and high addition were 3.256 mmo L/L and 3.004 mmo L/L, respectively. Only high addition had obvious influences on HDL-C, 日which was 1.484 mmo L/L. In conclusion, perilla frutescens seeds duck sauce could prevent and cure atherosclerosis, reduce blood fat.