Research Of Methods To Produce Astragaloside Ⅳ And Cycloastragenol

Astragalus herbs contains many types of Astragalus saponin, and many of these saponins that have been identified can be separated from the Astragalus root. AstragalosideⅣ is one of the main active components. It can strengthen the immune system, protect the liver, eliminate free radicals and so on, so we always think it has high medicinal value. Cycloastragenol is extracted from the dried roots of 1 Astragalus or obtained by the hydrolysis of Astragaloside IV, and it is considered to be the star molecule to anti-aging. In this study, we make full use of the Astragalus herbs and make research about how to produce Astragaloside Ⅳ and Cycloastragenol. The purpose of this project is to build an environmentally friendly and economy Astragaloside Ⅳ production methods to make foundation of the realization of Astragaloside Ⅳ’s industrial production. Another purpose is to make research on the different methods that conducts to produce Cycloastragenol, and we can analyze how AstragalosideⅣ change to Cycloastragenol, at last we can find the best production methods.Firstly, compare the detection methods we have known such like HPLC-ELSD, HPLC-RI and TLC, and optimize the detection conditions Secondly, establish the method to produce AstragalosideⅣ:①Optimize the traditional solvent extraction method and select the best raw materials with highly Astragaloside Ⅳ. ②Then we can make use of microbial fermentation, and the fermentation medium was optimized:Astragalus powder 20 g·L-1, glucose g·L-1, yeast extract 5 g·L-1, (NH4) 2SO4 2.8 g·L-1, KH2PO4 4 g·L-1, MgSO4·7H2O 1 g·L-1, CaCl21 g·L-1, initial pH value of 5.0. At the same time, optimize the fermentation conditions:inoculated seed liquid volume ratio of 1%, temperature 28℃, rotating speed 120 r·min-1, Cycle 6 days. We select HPD-100 macroporous resin, then explore the optimal separation conditions. At last we obtain product which purity is about 11%, and the process yield of 95%. The we use the silica gel column chromatography to separate the product with the purity of 86.5%and the yield of this step was 70.8%. Recrystallized from methanol the purity can achive more than 98%.Finally, explore the cycloastragenol production methods: ①optimize acid hydrolysis method:conversion rate of Cycloastragenol rearch more than 60%, but the method is with high energy consumption and serious pollution, especially for thehigh Astragaloside waste. ②Commercial enzyme hydrolysis method:the best commercial enzyme is from Silver Sino Biochemical Co., Ltd,andwe optimize hydrolysis conditions of this β-glucosidase. But the Cycloastragenol conversion was about 10%, then we simply explores the enzymatic inhibition factor. ③We use Trichoderma viride and Aspergillus carbonarius mixed to ferment Astragalus herbs Optimized the medium and fermentation conditions, and the cycloastragenol conversion raise to 26%. But it is difficult to make improve any more. This method which has many unresolved issues has many advantages and deserve further exploration.