| Coreopsis tinctoria Nutt.(CT, Asteraceae) is a widely distributed medicinal plant in southern region of Xinjiang Uygur Autonomous Region in China. Method: The research used by product of Coreopsis tinctoria Nutt. as raw material. And in this study, Coreopsis tinctoria Nutt. as raw material for processing in tea extraction process, clarification and concentration process, the drying process, inhibit oxidation and the role of hyperlipidemia were studied in vivo and in vitro. With extraction rate of flavonoids, the main factor influencing the extraction which included ratio of water to tea, extraction temperature, extraction time and extraction times were studied by using water extraction. In the complex enzyme assisted extraction, extraction temperature, extraction time and dosage of enzyme were the main factors.The complex enzyme assisted extraction technology and water extraction method were optimized. The experiment of clarification was conducted by three kinds of clarifying agents was analysed respectively including Gelatine, Arabic gum and CMC-Na. The effects of transmittance, flavonoids content and chromatic aberration L value were studied. Concentrated technology was used by the method of vacuum evaporation to concentrate the extracting solution. Drying technology: With extraction rates of water, flavonoids and free amino acids as indicators, two ways for drying(spray drying and vacuum freeze-drying) were compared, and then to evaluated the optimal technology of drying, and to evaluate the effects of product sense and rate of flavonoids with the different dosages of β-CD. The last, the antioxidative activity of Coreopsis tinctoria Nutt. instant tea was measured by a system of assay in vitro, including the total reducing power, the superoxide anion radical-scavenging activity, the hydroxyl radical-scavenging activity and the DPPH radical-scavenging activity. The haemolysis of rat erythrocytes and the generation of MDA of mice organ tissue were also detected. Furthermore, we evaluated the activity of its lowering serum lipid using high fat diet-induced hyperlipidemic rat model as well anti-oxidative activity in vivo.Result: The results shown that the optimal parameters of the complex enzyme assisted extraction were as follows the rate of composite enzymes(cellulases and pectinase) was 0.6%, hydrolysis temperature 60°C and hydrolysis time 90 min, and the extraction rates of flavonoids was 16.13%. The optimal parameters for enzyme pretreatment and water extraction were as follows: the ratio of water to tea 1:60(w/F), extraction temperature 60°C, extraction time 60 min. Under these conditions, the extraction rates of flavonoids was 17.86%. The optimal method of clarification was the of Gelatine which has the better clarifying effects and could effectively keep the active component such as the rate of flavonoids. The two methods of the two ways for drying(spray drying and vacuum freeze-drying) both had advantage. however, considering the drying efficiency and cost of production factors, spray drying has obvious advantages. Coreopsis tinctoria Nutt. instant tea shown the excellent antioxidant activity in the multi-test systems, including a quite strong concentration-dependent inhibition of its reducing property, DPPH radical, hydroxyl radical and superoxide radical. The present study demonstrated that Coreopsis tinctoria Nutt. instant tea have the protecting properties against erythrocyte haemolysis induced by H2O2 appeared to be dose-dependent and inhibit the generation of MDA in the liver, pancreas and kidney tissues of mice in dose-dependent manner. The experiment demonstrated that Coreopsis tinctoria Nutt. instant tea could ameliorate hyperlipidemia by reducing serum TC, TG and LDL-C levels and also enhanced GSH-Px activity in high-fat diet-induced hyperlipidemic rats. The research results indicated Coreopsis tinctoria Nutt. instant tea could inhibit lipid peroxidation and scavenge free radicals, and also have a stronger lipid regulating effects in vitro and in vivo. |
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