Synthesis And Properties Of St-DVB-MAA Polymer Micro-spheres SPE Packing

Sample pretreatment methods have great influence on the precision and accuracy of analytical results.Solid phase extraction as a convenient, fast and low consumption pretreatment method played an important role in analytical chemistry sample preparation. Solid phase extraction packing is an important component for solid phase extraction column. The solid phase extraction packing which have lipophilic fat and weak cation exchange performance was synthesized by the means of suspension polymerization, and the structure, morphology and properties of the packing were characterized. In the experiment,styrene and methacrylic acid were used as raw materials, polyvinyl alcohol as dispersing agent, and calcium carbonate powder as inorganic dispersants; Benzoyl peroxide were choiced as the initiator,and divinyl benzene as crosslinking agent to synthesize St–DVB–MAA polymer filler. The FT – IR, SEM and EDS methods were used to detect the structure, composition and morphology of the composite material analysis.The specific surface area and pore size distribution of the packing were determined by nitrogen adsorption method. Solid phase extractionl column which filled with St – DVB – MAA polymer extract cyanazine,simazine,atrazine and terbuthylazine four kinds of triazine pesticide in water and test the contents using the method of HPLC. Inspect the best condition of extraction: The sample p H=7, ammoniation of methanol as elution solvent, and the liquid flows through the column velocity was 1m L/min. The adsorption capacity of solid phase extraction packing of 4 kinds of compounds were determinated:: cyanazine,simazine,atrazine and terbuthylazine were respectively 27.1 mg/g,28.0 mg/g,28.3 mg/g and 27.5mg/g. There was a good linear relationship between the chromatographic peak area and the concentration of the four kinds of compounds in the range of 1.0 – 100μg /m L,with correlation coefficient(R) 0.9984, 0.9979, 0.9976 and 0.9966 respectively. Relative standard deviation was 5.2% – 8.1%,5.8% – 7.8% and 5.8% – 7.2% and 5.4% – 7.3%; Recovery range of 79.89% – 80.11%, 84.40% – 85.42% and 84.40% – 85.76% and 85.98% – 86.36%; The minimum detection limit were 0.25μg/L, 0.33μg/L, 0.42μg/L and 0.58μg/L.