Study On Cinnamon Hypoglycemic Active Ingredieents Isolation And Structure Identification

Cinnamon (cinnamomum cassia presl) is a kind of camphor tree plant, it’s a food and drug resource. Its extracts were found to have the effect of lowering blood sugar for the past few years. This paper studies for extraction method, time, solvent and its volume fraction of cinnamon hypoglycemic active ingredient. We use ultrasonic method, hydraulic extraction and the multi-function extraction for extraction of cinnamon hypoglycemic active ingredients. Column chromatography was used for separation and purification of cinnamon hypoglycemic active ingredient. With ultraviolet spectrum, infrared spectrum, the mass spectrum and nuclear magnetic resonance (NMR), the combination of cinnamon is determined the molecular structure of the active ingredient. After that, cinnamon active ingredient is used for animal testing, and at the same time we analyze the mechanism of hypoglycemic. Finally we have carried on the cinnamon hypoglycemic active ingredients for animal testing.The main research results are as follows:1 Studies for extraction method, solvent and its volume fraction, and the time to the cinnamon hypoglycemic active ingredient, and carries on the orthogonal experiment. The experimental results show that the optimal extraction method is multi-function of extraction, solvent is 95% ethanol, extracting time is 2 h. These combinations are the optimized way of cinnamon hypoglycemic active ingredient’s extraction.2 Experiments show that good separation and purification of cinnamon extracts is column chromatography, hydroxymethyl chalcone’s extraction and separation of quantity was 65.4%. So we use column chromatography to separate and purify cinnamon hypoglycemic active ingredients.3 By comparing the inhibitory effect of four hydroxymethyl chalcone fora-glycosidase enzymes and nonenzymatic protein glycation, it can be found that the good inhibitory activity of chalcone compound retained two hydroxyl groups in A ring, while the other three chalcone compounds’ inhibiting effect are bad, because there is no hydroxyl groups in A ring. Therefore, it can conclude that the presence of hydroxyl groups in A ring improve inhibitory effect. The more number of hydroxyl groups, the better the results.