The Manufacture Technology Of Grifolafrondosa Polysaccharide And Its Function Of Falling Blood Glucose

This paper study by the process conditions of extracting polysaccharide fromgrifolafrondosa through single factor test and orthogonal test. This experiment selectedextract time, extract temperature, ratio of feed liquid and alcohol concentration asexamine factors, and selected polysaccharide yield as index.After single factor test andorthogonal test, this experiment got the best extract technology conditions: the extractsolvent was water, the material liquid ratio was1:20(W: V), the extract temperaturewas120℃, the extract time was3h, and the extract alcohol concentration was95%.Under the condition of the optimal extraction process, the grifolafrondosa coarsepolysaccharide yield was7.5872%.Using single factor tests and response surface experiment,this paper researched thebest purification process conditions with the sevage method to remove proteinimpurities in crude polysaccharide. There are four single factor conditions: thechloroform and n-butanol volume ratio of sevage reagent,the polysaccharide solutionand sevage reagent volume ratio, the protein shakes time and the frequency ofvibration.Through single factor tests, response surface tests and validation tests, thispaper got the best technology conditions to remove protein impurities in crudepolysaccharide: the chloroform and n-butanol volume ratio was4.02:1, thepolysaccharide solution and sevage reagent volume ratio was3.07:1, the vibration wavetime was25.6min, the deproteinization number was1times. In the bestdeproteinization condition， the deproteinization rate was37.95%, and thepolysaccharide content was78.32%.In further purification experiment, after screening， this experiment used theDEAE-agarose gel FF,because it has many characteristics, such as resistance to pressure,resistance to acid and alkali, and high velocity of flow. In the experiment, thepolysaccharide content in the product reached89.33%after the separation ofDEAE-agarose gel FF. With further separation by Toyopearl HW-75molecular sieveand high performance liquid chromatography method, this experiment got the singlepeak symmetry and the polysaccharide purity was97.9%. With the methods of infraredspectrum, gas chromatography, and nuclear magnetic resonance， this experiment hasconfirmed its structure.By high fat and sugar feed and intraperitoneal injection of chain urea with cephalosporins, this experiment got type2diabetes mice model. And then, thisexperiment has investigated grifolafrondosa,s function of falling blood glucose.Theresults of this experiment shew that the blood glucose and blood lipid levels increasedsignificantly in model mice after continuous dosing of eight weeks. grifolafrondosasporophore polysaccharide high and low dose group (300mg/kg and100mg/kg) has theeffect of lowering blood glucose and blood lipid of mice, and the mice appeared dosedependent phenomenon.