Detection Of Uranium, ATP And Manganese With Emission Spectrum Analysis Based On Bis-sulfosalophen Complexes

The determination of uranium, adenosine triphosphate(ATP) and manganese is of importance due to that these substances are closely related to environment and human health. Therefore, in this paper, we synthesized two new type of ditopic Schiff base ligands, Bis-sulfosalophen(BSS) and bis-phenanthroline-glutaraldehyde(BPG), and studied their structure and spectral properties. By using these ligands, we developed three new analytical methods for the determination of uranium, ATP, and manganese, respectively. One is a fluorescence method for the determination of uranium based on its effect on the florescence of BSS. Another is a resonance light scattering(RLS) method for the determination of ATP based on its combination with both BSS and gold nanopaticles(Au NPs) to cause the aggregation of Au NPs. The third is a RLS method for the determination of manganese based on its coordination reaction with both BSS and BPG to form a supramolecular polymer. These methods provide new strategy for the determination of uranium, ATP, and manganese.In the first part of this paper, firstly the properties and the research progress in analysis of uranium, ATP, Mn(II) are reviewed, then the properties and applications of salophen and its metal complexes are introducced. Meanwhile, the analytical applications of coordination supramolecular polymer, resonance light scattering and gold nanoparticles are also introduced.In the second chapter, we synthesized Schiff base ligands BSS and characterized its structure, then studied its application in the fluorimetric determination of uranium. The experimental results show that BSS has good water solubility. Its maximum fluorescence emission wavelength(λem) is at 447.0nm. The fluorescence intensity has a good linear relationship with the uranium concentration in the range of 0.1 to 1 nmol/m L with a detection limit of 0.03 nmol/m L under optimal conditions. The method has been successfully applied to detect uranium in real samples with the recoveries of 97.0 ~ 102.0%.In the third chapter, a RLS method for the direct detection of ATP in homogeneous solution without separation procedure is proposed. Bis-uranyl-Bis-sulfosalophen(BUBSS), a Bisnuclear uranyl complex synthesized by the coordination reaction of two uranyl ions with BSS, and anti-ATP aptamer modified gold nanoparticle probers(Apt-Au NPs) were prepared for the experiment. A BUBSS molecule is easily to specifically combine two ATP molecules to form a linear supramolecule. The formed supramolecule is easily to specifically bind two Apt-Au NPs further to cause the aggregation of the Apt-Au NPs, resulting in the production of strong RLS signal from the system. Based on these facts, the RLS method for the determination of ATP was developed. A linear range was found to be 15 to 500.0 nmol/L with a detection limit of 4.5 nmol/L under optimal conditions. The method has been successfully applied to detect ATP in real samples with the recoveries of 96.3-102.5%.In the fourth chapter, the formation of a manganese(II) coordination supramolecular polymer was studied by RLS spectra for manganese(II) detection. BSS and BPG(a ditopic bidentate ligand) were prepared for the construction of the supramolecular polymer. In a procedure of manganese(II) detection, manganese(II) ion reacts with BSS to form a binuclear manganese(II) complex. The binuclear complex then self-assembles with BPG to form the supramolecular polymer, resulting in a production of strong RLS signal. The amount of manganese was detected through measuring the RLS intensity. Under optimal conditions a linear range was found to be 0.5 to 50.0 ng/m L with a detection limit of 0.1 ng/m L. The method has been successfully applied to determine manganese in vegetable and tea samples with the relative standard deviations of less than 5% and the recoveries of 95.8 – 105.7%.