Changes Of Antioxidant Enzyme Activity And Methylation Status Of Apoptosis - Related Genes Induced By Cadmium In

Cadmium (Cd) is a non-essential organisms and serious harm of toxic heavy metals, which can enter the body through a variety of ways feeding organism, respiratory, skin infiltration of various organs of the body produce toxic effects in varying degrees. Toxicological effects of cadmium is also reflected in the organism can cause epigenetic changes, including genomic DNA methylation level, some gene promoter methylation status and antioxidant enzyme system and methyltransferase activity changes. At present the research cadmium stress organisms are more concentrated in aquatic organisms, less coverage of terrestrial organisms, invertebrate Drosophila as a model organism in the study of cadmium exposure of their physiology, biochemistry and other areas has little to people know. In this study, Drosophila melanogaster Canton-S strain as experimental material,Drosophila different concentration (0 mg/L,6.5 mg/L,13.0 mg/L,26.0 mg/L,52.0 mg/L) of cadmium stress treatment. Drosophila using spectrophotometry different days of age (1 d,10 d,20 d,30 d,40 d,50 d,60 d) to fly the body of superoxide dismutase (SOD), catalase (CAT) activity and malondialdehyde (MDA) content. And application-specific methylation PCR (MSP) method to explore cadmium on apoptosis-related genes in Drosophila melanogaster p53, tefu, damm, caspase-3 of DNA methylation status. The results are as follows:1.Cadmium-induced Drosophila SOD, CAT activity changes, and the trend was negatively correlated with the cadmium concentration. SOD and CAT activity under cadmium stress, the trends are very similar, and no significant difference between male and female change. Low concentrations of Cd-treated group (6.5 mg/L,13.0 mg/L) flies SOD, CAT activity in the general trend in the 1-40 d within the growth with gradually rising day-old flies. Which rise 1-20 d within the more significant, but the first 1 d data showed no significant difference between the concentrations. When the incubation time reached 40 d, SOD activity reached the maximum, this time between each concentration group were significantly different. Article 50 d test results show, this time SOD activity versus 40 d test data in terms of significantly reduced, compared with CK group, the treatment group showed significant differences among high concentration group were significantly difference. Article 60 d when testing found that low concentrations of all deaths in Drosophila. High concentrations of cadmium-treated group (26.0 mg/L,52.0 mg/L) activity at low concentrations similar trend, but with the control group was significant difference. CAT activity was significantly analysis showed similar and SOD, but among the various concentrations were significantly different when CAT at 20 d after detection. And SOD in the first 10 d after they experienced a significant difference, suggesting that SOD may be more sensitive to stimulation caused by cadmium. Drosophila MDA in the process also changed significantly throughout the development cycle, the control group, a low concentration (6.5 mg/L,13.0 mg/L) and high concentration (26.0 mg/L, 52.0 mg/L) MDA content of cadmium treated flies with increasing days of age increases, and treated with increasing concentrations of Cd, MDA concentration also increased, but the increase is waning.In summary, this study analyzed, the activity of antioxidant enzymes of Drosophila cadmium SOD CAT and MDA content, the results show that cadmium-induced changes in antioxidant enzyme activity there is a certain measure dependent.2. DGE according to preliminary laboratory results, we selected four and senescence and apoptosis in Drosophila genes closely related to p53, tefu, damm, caspase-3.PCR method using specific methylation, explore the four gene methylation status in the role of cadmium. The results show, p53, tefu, damm 3 genes induced by heavy metals cadmium, the emergence of the corresponding methylated bands, caspase-3 gene did not show methylation bands. We speculate, p53, tefu, damm 3 genes induced by heavy metals cadmium, the potential of methylation phenomenon. With this result we Drosophila promoter CpG islands 5’cytosine in the role of bisulfite, and indeed the existence of the situation is not changed, that may have occurred DNA methylation phenomenon, before this conclusion the reported absence of Drosophila melanogaster epigenetic imprinting this conclusion is different.The use of model organisms of heavy metal stress inducible enzyme activities and content-related changes in the product of their antioxidant enzyme system, to explore and induced oxidative damage and epigenetic changes resulting potential regulatory mechanism for more depth cadmium pressure stress response in Drosophila cells the molecular mechanisms of apoptosis. And later explore the influence of DNA methylation in Drosophila apoptosis in DNA methylation levels provide research support for fruit flies depth study of DNA methylation system and environmental factors induced table genetic Variation Viewpoint experimental. Basis for the study of human epigenetic factors and their relationship with the environment and other aspects of opening up new ways to circumvent epigenetic changes caused by environmental factors may cause the future of ecosystem impacts and risks theoretical basis, but also for heavy metals and diagnosis and treatment of human diseases caused provide new ideas and experimental basis.