DNA Kinematics Modeling And Error Real - Time Calibration For Visual Nanostructures

DNA carries too much information and significance of life, and the research in the life science has a crucial role. AFM (atomic force microscope) is an important nano-manipulation tool, which plays an important role in the DNA operation. AFM is the main scan imaging and operating tool. The normal procedure is scan-operation-rescan, and AFM firstly scans an image and then operates in the image, and finally rescans to test and verify, so its efficiency is relatively lower. In order to overcome the defect, the immersive operating system appears, which is based on the visual and force feedback. According to the force analysis, the image of target object is rebuilt, and at the same time, the tip of AFM starts to scan and update the user interface to achieve real-time visual feedback effect. But these are mainly for the kinematic model of rigid objects such as nanoparticles and nanorods, and lack of flexible objects, such as DNA.This paper contains five chapters.The second chapter is mainly research and simulate about the model of DNA. According to DNA stretching curves, the DNA is simplified into spring-mass. Based on that, we go on the force analysis、equation establishment and discussion, and finally get the matlab simulation result to verify its validity. The program is listed in the appendix. The third and the fourth chapters mainly display the DNA reconstruction process. The third chapter solves the problems of the pretreatment of DNA. In this chapter,we describe some of the basic operations of mathematical morphology and the detailed mathematical explanation, and then go on the corresponding erosion operations, and finally get a satisfactory corrosion result which is verified by a new and fine tip (expansion effect is relatively little). The fourth chapter is mainly about the digital image processing of DNA. Here the use of many methods including the image preprocessing, smoothing, filtering, thresholding segmentation, edge detection and extraction, skeleton segmentation, eventually geometric characteristics data of the DNA are acquired. In practice, we may encounter many errors because of the inaccuracy and therefore require a suitable method for correction. The fifth chapter is to establish a software interface for DNA operation with partial scan method for correction. The key flow charts of the program are designed, and the results of each operation are shown separately.At last, the corresponding summary is made. The method based on DNA model and partial scan method for correction has a certain guiding significance for the development of DNA operations.