Preparation Of Hydrogen Dioxide Biosensor Based On Graphene Films-Nanostructured Mesoporous Platinum

Graphene was a kind of new carbon materials after the fullerene (C60) and carbon nanotube (CNT). The thickness of graphene was only one carbon atom, which was only about0.335nm, which was the thinnest material known in the world. It has many excellent properties, such as good thermal conductivity, good electrical conductivity, very high mechanical strength and unique electrical properties. Although graphene has many excellent characteristics, it reunite easily because of the π-π superpositionbetween graphene layers.So its controllability was defected, such as the fabrication of graphene film and biosensors based on graphene film.At present, there have many ways to produce conductive grapgene film, such as spin-coating ethod,vacuum filtration method and self-assembly method.Self-assembly method is easy to make oxide graphene films and the area of which is controllable,and the film has good controllability and homogeneous. The only defect is the conductivity of the films greatly reduced because of using oxide graphene as raw material at present.Hydrogen dioxide is an important chemical material. It can take occur reduction reaction or oxidation reaction under different conditions. Hydrogen dioxide is the production of catalysis reaction produced by enzyme. It is also a important material in industrial manufacture, processing food, clinical medicine and environmental analysis. But using hydrogen dioxide exceed standard is bad to the health of people. So it has important significance to detect hydrogen dioxide quickly and accurately. At present, the detection methods of hydrogen dioxide mainly contains fluorescence method,ECL(electrochemical luminescence)method and electrochemical method.The electrochemical detection of hydrogen dioxide is mostly did by biosensor modified with enzyme.It is complex to made the enzyme electrode, and the enzyme may drop from to the substrate, leaing to degenerate and inactive, so it is limited the application of biosensors.It was found that poriferous nanoplatinum has good catalysis property because of its higher specific surface area, especially to the hydrogen dioxide. It also has higher sensitivity. Poriferous nanoplatium is a kind of material with special structure and function, because its’ metal framework usually is innanometer size, and there were many connected hole inside. This structure makes it has high specific surface area, low proportion and economic. So it has extensive application in many fields, such as catalysis, energy storage and biosensors.In this paper, we prepared graphene nanosheet by electrochemical reduction method, and then used the graphene nanosheet as raw materials to obtain a stable, uniform, and controllable conductive graphene films in the liquid-liquid phase surface by means of self-assembled, and then transferred the film to the surface of glassy carbon electrode to prepare graphene film electrode. In order to impart special properties and application to the graphene film electrode,we modified nano mesoporous platinum on the surface of grapheme film electrode, and studied the electrochemical catalytic reduction of the composite electrode to the O2and H2O2, developing a kind of nonenzymic sensor without medium which used for detecting H2O2. This research provided new method and technology to research of poriferous nonenzymic sensor.