| Objective: Determination of skeletal muscle hypertrophy and atrophy through differenttraining modes regulatory factor changes, and skeletal muscle weight and cellular changes inthe cross-sectional area, combined with the above changes, further explained the differentsports training on skeletal muscle hypertrophy and atrophy the impact of the molecularmechanisms of effective theoretical basis for the study of the molecular mechanisms ofskeletal muscle hypertrophy and atrophy.Methods: study selected508-week-old male SD rats were randomly divided into a quietgroup (C), endurance training group (T), the anti group training group (R), the injury traininggroup (I) and hind limb overhanging group (H),10in each group. Adaptability startedtraining two weeks after training. The first nine weeks of the Group H began formal training.Group E, incline to0°, speed of30m/min,1times/day,60min/6days/week;Group R, the second week of rat weight is30%of the body weight, after increasing loadper week for25%of the body weight, weight increased to200%of the body weight of ratswill no longer increase the load.6days/week,4/day,3times/group. Each time you climbto the top of ladder to rest for1min, rest between sets3min. Ladder high1m per grade ladderare separated by2cm. Training ladder placed tilted85o.(Loaded rat tail weight placed on thebottom of the ladder apparatus, rats independent upward crawl driven other means, ifnecessary.);Group I, speed16m/min, slope-16°,1times/d,45min/d,6d/week.Group H,50×50×50cm squirrel cage reared separately, free of drinking water. Thespecific method is as follows: with a thick towel to wrap in rats exposed tail. The rat tail Washwith soap and water, dry naturally or use a hair dryer to dry. Respectively benzoin tincture androsin tincture for smear tail. When dry, the wale direction, with the tail width two calico bandare attached to the tail, respectively above and below (white belt to grow for one-third of thetail). Then surround from the root of the tail with surgical tape to the distance of the tip of thetail at5cm. As the tail with the isolation of white cloth, with surgical tape wound around onlyafter contact with tape on both sides, up and down both sides of the site as the next time you replace the tape pasted. Finally, on a the rotating key ring grow a tail of white cloth with theDepartment of360o level, to facilitate the free movement of rats. The overhanging height ratsalways maintain bow position is appropriate (head and ground into an angle of30℃).Replaced weekly paste parts.Stop training the next day, take the SD rat gastrocnemius detection gastrocnemius musclearea, and p-Akt protein expression by immunohistochemistry. Western blotting was measuredMuRF1protein expression.The results:Weight body weight of rats12weeks showed a significant difference compared to thefirst week weight., R group rats body weight increased by a big margin, with C rats weightincrease was significant difference; rats E weight gain or than C group showed significantdifferences compared with C group; group I mouse weight gain or degree of declinecompared with the E group, C group showed a significant difference; decline in H rats anincrease or degrees compared to group I, group C showed a significant difference. Nine weeks,the rats I weight than eight weeks significantly decreased.Rat gastrocnemius muscle cross-sectional area, R group was significantly higher than ingroup C were sex (P <0.01). E and C groups no significant difference. I was significantly lessthan the C group (P <0.05). Group H was significantly lower than that in group C (P <0.01).The rat gastrocnemius Weight, R group was significantly greater than in group C (P<0.01). E and C groups no significant difference. I was significantly less than the C group (P<0.05). Group H was significantly lower than that in group C (P <0.01).Rat gastrocnemius p-AKT protein expression R group was significantly higher than thatin group C (P <0.01). Group E was significantly higher than that in group C (P <0.01). Thegroup I was significantly higher than that in group C (P <0.01). H and C groups no significantdifference.Rat gastrocnemius MuRF1protein expression, R group is significantly lower than that ingroup C (P <0.01). E and C groups no significant difference. The group I was significantlyhigher than that in group C (P <0.01). Group H was significantly higher than the C group.Conclusions:1.Anti-group training can lead to the body weight of rats, gastrocnemius weight,increased cross-sectional area of gastrocnemius muscle, and the ability to activate skeletalmuscle hypertrophy factor Akt activity, thereby enabling the gastrocnemius hypertrophy.2.Endurance training result in weight loss in rats, gastrocnemius weight and cellcross-sectional area, enabling Akt phosphorylation, of the MuRF1no significant impact.3. Injury training lead to slow weight increase in rats and enables gastrocnemius weight,gastrocnemius muscle cross-sectional area decreases and the activation of Akt and MuRF1 activity. The movement mode rat skeletal muscle hearing adaptive mechanisms may beinvolved in a variety of signal transduction pathways.4.Hind limb overhanging the rat body weight, the weight of the gastrocnemius,gastrocnemius muscle cross-sectional area decreases, and be able to activate the skeletalmuscle atrophy factor MuRF1activity, rat gastrocnemius muscle atrophy.5.Different training modes have different effects on MuRF1expression, resistanceexercise and endurance exercise its significant impact hind limb overhanging MuRF1expression, the impact of the injury in training MuRF1expression. |
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