Effects Of Voluntary Exercise On Learning And Memory,Notch Signal Pathway Related Gene Expression Of Young Mice

Learning and memory is one of the important function of the brain, it is important basis to human to participate in social activities. But the ability of learning and memory will be reduced (such as Alzheimer’s disease) with age and other factors, which affect people’s normal life. More and more researches shows that the appropriate exercise can improve the ability of learning and memory, and promoting people’s cognitive function, and prevent Alzheimer’s disease. Exercise can promote the ability of learning and memory, but the specific mechanism is not very clear. A lot of animal experiments show that suitable exercise can improve brain derived neurotrophic factor, and improve the ability of learning and memory. Also experiments on animals show that suitable exercise can promote neural stem cells proliferation and differentiation, and producing new nerve cells, improving the synaptic plasticity, which can enhance the ability of learning and memory. Research shows that BDNF have benefits for the neuronal proliferation, differentiation and growth. And more and more evidence that there is a relevant between the proliferation and differentiation of neural stem cell and Notch signaling pathways. Therefore, this study was about voluntary exercise promoting learning and memory. And this study has taken BDNF and Notch signaling pathways to discuss the relation of the learning and memory and sport. At the same time, the study analysis and discussion correlations between BDNF and Notch signaling pathways.Objective:This study focus on the effects of voluntary exercise on learning and memory, BDNF gene expression and the Notch signaling pathways with voluntary exercise. And this paper discusses the possible mechanism that exercises improve the ability of learning and memory.Methods:We can choose12mice from30male C57BL/6mice which do voluntary exercise.12mice are randomly divided into exercise group (n=6) and control group (n=6), while the exercise group do voluntary exercise from Monday to Sunday for8weeks. At the8th week,12mice all participated in the text of the water maze.After24hours, we can take hippocampus through broken the neck. And text the expression of BDNF mRNA, Notch mRNA, PS-1mRNA, Hes-l mRNA by RT-PCR.Results:(1) According to the date in the Morris water maze test, sport group performed better the control group.(2) voluntary exercise raised the expression of BNDF mRNA,Notch-l mRNA, PS-1mRNA,Hes-l mRNA.Conclusions:(1)8weeks of voluntary exercise improve the C57BL/6mice of learning and memory through the text of Morris water maze.(2)8weeks of voluntary exercise increase the C57BL/6mice of BDNF gene expression, illustration that there is a relevant between learning and memory and BDNF.(3)8weeks of voluntary exercise increase the C57BL/6mice of Notch-l,PS-land Hes-l gene expression, at the same time. Explain,it is matters that Notch signalling pathways is to learning and memory.(4)8weeks of voluntary exercise improve the C57BL/6mice of learning and memory. The mechanism may be:①voluntary exercise raise the expression of BDNF mRNA,caused increasing of the Notch-1mRNA,activate Hes-1. Those can adjust the nerve stem cell proliferation, which can regulates synaptic plasticity,and then, improve the ability of learning and memory.②voluntary sport may increase the expression of PS-l mRNA increase the NICD, up regulation Hes-l mRNA, affect neural stem cell proliferation, change the synaptic plasticity, and improve the ability of learning and memory.