The Effect Of Exercise On Mesenchymal Stem Cell's Proliferation And The Effect Of Their Proliferation And Osteogenic Differentiation Of Chinese Medicine

ObjectiveTo observe the effect of exercise training on the proliferation of MSCs,and to research the effect of Zheng-Shi-San-Hao liquor extract on proliferation and osteogenic differentiation of rabbit MSCs.Methods1 Devide the eight rabbits into two groups:the quiet group and the exercise training group.Make the rabbits of exercise training group run at the speed of 25m/min for 60 minutes a day,five days a week for four weeks,the quiet group is feed as usual.2 Take bone marrow from rabbit tibia of the two groups sepestertly.MSCs are isolated by the method of density gradient centrifugation with stick to plastic then cultivante.We can observe the effect of exercise training on MSCs by means of cell morphology and MTT evaluation for the 2-8 days.3 We withdraw Zheng-Shi-San-Hao liquor and study P2 generation,divide it into 5 groups(The blank control,conditional group,Zheng-Shi-San-Hao liquor groupl,Zheng-Shi-San-Hao liquor group 2,Zheng-Shi-San-Hao liquor group3)and cultivante them and observe the effect of Zheng-Shi-San-Hao liquor on MSCs by means of cell morphology and MTT evaluation.4 Study P2 generation of MSCs and divide it into 5 groups(The blank control group,conditional group,Zheng-Shi-San-Hao liquor groupl,Zheng-Shi-San-Hao liquor group 2,Zheng-Shi-San-Hao liquor group3) and cultivante them seperately,observe the effect of Zheng-Shi-San-Hao liquor on osteogenic differentiation of MSCs by means of ALP activity determination. Result1 According to the statistic analysis,there are no conspicuous difference of the proliferation of rabbit MSCs between the quiet group and the exercise training group by means of MTT evaluation.2 According to the statistic analysis,there are no conspicuous difference between blank control and conditional group,while there are difference between Zheng-Shi-San-Hao liquor groups of different concentration and conditional group.3 The result of ALP activity determination is:Zheng-Shi-San-Hao liquor group3＞conditional group＞Zheng-Shi-San-Hao liquor group 2＞Zheng-Shi-San-Hao liquor group 1＞blank contrast groupConclusion1 The proliferation of rabbit MSCs can not be affected by our exercises training procedures,running at the speed of 25m/min for 60 minutes a day,five days a week for four weeks.2 The Zheng-Shi-San-Hao liquor has bidirectionally modulatory effect on MSCs.The group of high concentration can inhabit the proliferation of the rabbit MSCs while those groups of lower concentration can promote the proliferation of the rabbit MSCs.3 We have demonstrated that some concentration of Zheng-Shi-San-Hao liquor contains ingredients that can promote the osteogenic differentiation of MSCs.