Table Epigallocatechin Gallate On Tumor H ₂₂ Experimental Observation Of Tumor Growth In Mice And Antioxidant Effect

Objective To explore the effect of epigallocatechingallate (EGCG) on the tumor growth, antioxidant effect, protein expression of Bcl-2and Bax in H22mice. In addition, antitumor effects and ability of regulating immune function of EGCG were both expected to observe.Method KM mice were randomly divided into six groups: the control group, the tumor-model group, low-, medium-, and high-dose EGCG group and the5-Fu positive control group. Then transplanted tumor model of H22mice was established. The second day the model group were given0.5mL soybean salad oil (ig), the EGCG groups were treated with EGCG (ig) of different dosage consecutively and the5-Fu group were given5-Fu (ip)20mg/kg·d-1once each other day. They were all executed after14days. Tumor, which was initially weighed and completely stripped, was used to calculate the tumor inhibition rate. Simultaneously, the expression of Bax and Bcl-2in tumor tissue was determined by immunohistochemistry. The concentration of superoxide dismutase (SOD), malonyl dialehyde (MDA) and glutathione peroxidase (GSH-Px) in blood plasma were also detected with the test kit.Result Tumor weight in EGCG groups was increased slower than the model group, and the inhibition rate was raised in accordance with the dosages of EGCG. The inhibition rates of low-, middle-, high-dose EGCG groups were25.572%,35.367%and44.52%respectively. Furthermore, the viscera index of the thymus in EGCG groups had risen and had dramatic difference from the model group and5-Fu group (P<0.05). The viscera index of the spleens in EGCG groups had risen and had dramatic difference from the model group (P<0.05). Moreover, the difference between middle-, high-dose EGCG groups and the5-Fu group was dramatic (p<0.05). Compared with control group, GSH-Px and SOD activity tend to increase and MDA content was a downward trend in different EGCG groups (P<0.01).Conclusion Each group of EGCG inhibited tumor growth possessing with higher tumor inhibition rate. The anti-tumor mechanism of EGCG could be relavant with decreasing of protein expression of Bcl-2, increasing of protein expression of Bax, and enhancing of level of oxidation resistance.