Dietary Intake Surveys Of Patients And Correlation With Vitamin D Receptor Gene (rs2228570) Tourette Syndrome

Part I Evaluation Of Diet Quality Of Children With Tourette Syndrome In ChinaObjective To investigate nutritional condition,to know dietary and snacks intake status of Tourette syndrome(TS) in Shandong province and to conduct patients to improve the quality of dietary intake and choose healthy snacks.Methods A total of48patients diagnosed with TS were recruited for the study. Self-designed questionnaires were used to know dietary behavior. Z scores were used to assess the nutritional status of patients;24h dietary recall was used to investigate the dietary intake;the Nutrient Adequacy Ratio(NAR)and Dietary Diversity Score(DDS) were used to evaluate dietary quality. Meanwhile the status of snacks intake was investigated.Results1. In the respect of dietary behaviour,10.42%TS patients had eating disorders and52.08%TS patient prefered eating fired food.2. In the respect of nutritional status,35.42%of TS patients were overweight,14.58%of TS patients were undernutrition;8.33%of TS patients were emaciation.3. In the respect of nutrient intake, the mean of energy intake among adolescence and juvenile was low compared with recommended nutrient intake, the proportion of carbohydrate, protein and fat was inappropriate in the aspect of providing energy. The intake of vitamin A, vitamin Bl,vitamin B2,vitamin C, calcium and zinc were insufficient, the nutrient adequacy ratio were0.68,0.78,0.67,0.78,0.37and0.71respectively, the intake of vitamin E was excessive, the nutrient adequacy ratio were1.23.4. In the respect of DDS, the mean scores of DDS was5.21.5. In the respect of snacks intake,56.25%of the TS patients would influence the appetite of dinner due to eat snacks,54.17%of the TS patients didn’t eat nuts at all,"Limit consumption" coolers as snacks were most used among66.67%TS patients.Conclusion There are some unhealthy dietary behaviors and habits among TS patients.The prevalence rate of malnutrition among TS patients is high, malnutrition and over-nutritional symptoms coexist.Dietary nutrients intake is not appropriate, level of dietary diversity is low and TS patients choose snacks that are no suitable to them. So TS patients and their patients should accept food nutrition guidance to improve the structure of dietary intake and choose suitable snacks. Part II The Transmission Disequilibrium Analysis Between Vitamin D Receptor Gene Polymorphisms(rs2228570) And Tourette SyndromeObjective To analyze the association of vitaminD receptor(VDR) gene FokI polymorphism with Tourette syndrome (TS).Methods A total of186patients diagnosed with TS and their parents were recruited for the study. The genetic contributions of the VDR gene FokI polymorphism were evaluated using TaqMan real-time polymerase chain reaction. Haplotype relative risk (HRR) and transmission disequilibrium test (TDT) were used to analyse them of VDR gene FokI polymorphism with TS.Results1. The Hardy-Weinberg Equilibrium Test The genotype frequency distributions of patient and parent group were not deviated from the Hardy-Weinberg equilibrium (P>0.05), and it indicated these samples were suitable for the genetic analysis.2. The Haplotype Relative Risk (HRR) Test HRR test revealed that there was no significant associations between the VDR gene FokI polymorphism and TS(x2=0.35,P>0.05;x2=1.62,P>0.05).3. The Transmission Disequilibrium Test(TDT) TDT revealed that there was no significant associations between the VDR gene Fok I polymorphism and TS(x2=0.33,P>O.05)Conclusion There is no significant association between the VDR gene Fok I polymorphisms and TS.