Pharmacokinetics Zaoren Sedative Side Dynamics

Prescription of Jujube benevolence nerves(PJBN) are commonly used for clinical treatment of insomnia, composed of three ingredients,there are zizyphus jujube kernel, fructus schisandrae (main) vinegar, salvia miltiorrhiza.Prescription compatibility mechanism research is the key problem.PJBN’s pharmacokinetic study to reveal its compatibility mechanism is valuable.This topic takes Spinosin, ferulic acid, tanshinol, protocatechuic acid, protocatechuic aldehyde, fructus schisandrae alcohol and deoxyschizandrin as research object, do pharmacokinetic studies about them, and try to preliminary discussion from the mechanism of this prescription.The main research contents and results are as follows:1.Take PJBN as the research object, by the method of HPLC,measure the content of7kinds of effective components simultaneously. Results show that the precision of the instrument is good, the method of strong specificity, the composition of sample recovery rate is over95%, the reproducibility and stability of RSD is less than2%.2. To establish HPLC detection at the same time in rat plasma index component content of7kinds of methods By researching methodology, the linear relationship of7kinds of ingredients is good, high in the low concentration of three recovery rate were over75%, daytime days precision stability of RSD were below10%, conform to the requirements of the biological sample detection3. the above mentioned methods to the jujube benevolence nerves extract lavage component of the indexes in rat plasma after the treatment of pharmacokinetic process was studied, according to the results of jujube benevolence nerves party extract main metabolic components in plasma still exist in prototype form The average plasma concentration of pinot element in5h to spike after the treatment, and after a slow metabolism in the body Fructus schisandrae alcohol armour and fructus schisandrae armour medicine bimodal curve are present, when prompted the two ingredients may exist in the process of absorption of liver-bowel loops or gastric bowel loops, etc The drug pharmacokinetics result is expected to be in jujube benevolence nerves and its preparations can provide reference for pharmacokinetic study.4. Study jujube benevolence nerves of single herbs and medicine to extract lavage in rat plasma after dosing pharmacokinetic process of each index component and comparing with the compound Results show that the compound of pinot element in ti/2ka extended obviously, increase significantly Cmax, AUC(0-t) increases, explain its absorption is slow, increase bioavailability, better able to play a sedative hypnotic pharmacological effects, Cmax and AUC(0-t) are obvious rise, increased the concentrations in the plasma of rats with V1/F and CL/F decreased significantly, showed that the composition of=compound have synergy effect Fructus schisandrae alcohol armour t1/2ka and AUC(0-t) increased significantly, that formula to improve the blood concentrations of fructus schisandrae alcohol armour, and prolong its role in the body. May extend its calming effect on the central nervous system, increasing the protection of the liver The results of the experiment, from the perspective of pharmacokinetics of compound compatibility provides reference meaning.