Kochia Child's Active Ingredients Influence HaCaT Cell Cycle

Objective:Using in vitro cell culture experiments, the main effective components of the belvedere fruit HaCaT cell proliferation and cell cycle, explore its action mechanism for the treatment of psoriasis.Methods:The belvedere fruit main effective components mixture concentration of different drugs work according to the proportion of1:2,48h, role respectively in HaCaT cells with methotrexate (5ug/ml) as positive control group, without drugs as the negative control group. Determined by MTT colorimetric method is used to determine the main effective components of HaCaT belvedere fruit cell proliferation activity of influence efficiency; Flow cytometry analysis in belvedere fruit effect of effective components of the cell cycle characteristics.Results:1、Methotrexate group OD value and active ingredients belvedere fruit saponins IC25μmol/L group, there was no significant difference (P>0.05)2、Oleanolic acid had no effects on HaCaT cell proliferation;3、The rest of the saponins IC concentration groups have different degree of inhibition of HaCaT cell proliferation, and have concentration-response relationship, to increase the concentration dependence. Cell proliferation OD value between different concentrations was statistically significant (P <0.01)4、flow cytometry to detect the cell cycle of methotrexate group Lin OD value and active ingredients belvedere fruit saponins IC25um ol/L group, there was no significant difference (P>0.05).5、concentration of the rest of the group has the cell cycle in GO/G1phase cell proportion increase, and the S phase cell proportion reduce, the cell cycle arrest in G1phase.Conclusion:Belvedere fruit saponins in IC can inhibit HaCaT cell proliferation, and cell cycle arrest in G1phase, the mechanism may be related to affect the cell cycle.