| Objective:To study the high protein, high calcium chicken gouty arthritis induced by feed ratio of the animal model of the way and. part of the action mechanism of the model of Juan bi Li jie cleaning on experimental chicken gout,Methods:The first part,the animal model establishment:choose160healthy age of30days, male xiang yellow chicken, weight (500±50) g, were randomly divided into:the blank group (hereinafter referred to as group A) with normal feed, model1group (hereinafter referred to as the group B) with40.19%protein content,10.32%calcium content in the feed. Model2group (hereinafter referred to as group C) to50%protein content,9.17%calcium content in the feed; Model3groups (hereinafter referred to as the group D) to55.2%protein content,6.48%calcium content in the feed to carry on the induction, each group40, the daily limit of100ml of drinking water, feeding1D,7D and14D and21D randomly selected from each group of10only observe the morphology of chicken, chicken ankle weeks diameter, extract the wings under the venous blood detection of blood uric acid level, take ankle synovial executed, then observe the pathological changes.The second part, the experimental drug intervention:choose160healthy age of30days, male xiang yellow chicken, weight (500±50) g, were randomly divided into:blank group(hereinafter referred as group A, model group (hereinafter referred as group B), Chinese medicine Juan bi Li jie cleaning group (hereinafter referred as group C), allopurinol group (hereinafter referred as group D), each group40. In addition to the group A, the rest three groups A conclusion according to the experiment chicken gouty arthritis animal model is set up. A group and B group was given saline, group C was given Juan bi Li jie cleaning aqueous solution, group D was given allopurinol aqueous solution to fill the stomach, twice daily.7d,14d and21d three phase points in observation of chicken, feeding1d,7d,14d and21d of each group of randomly selected10only observe the morphology of chicken, chicken ankle weeks diameter, extraction under the wings venous blood testing blood level of uric acid and XOD level, take ankle synovial executed, then observe the pathological changes.Results:The first part:the animal model establishment::the first part of the animal model of group C compared with B, D form two groups observed score (see table1), joint weeks size comparison (see table2), the number of neutrophils in the articular synovial comparison (see table3), joint synovial blood vessels in the number of comparative (see table4), blood uric acid levels (see table5),7d after statistics analysis when there was no statistically significant difference (P>0.05),14d the difference was statistically significant (P<0.05);21d the difference has significant statistical significance (P<0.01). C way morphology observed score comparison (see table1), joint weeks size comparison (see table2), joint synovial neutrophil number comparison (see table3), vascular number comparison in synovial joints (see table4), blood uric acid levels (see table5),7d and14d after statistics analysis,21d had significant difference compared with1d statistically significant (P<0.01),14d and7d comparative difference was statistically significant (P<0.05),21d and7d,14d compare difference has statistical significance (P<0.01).The second part:group C compared with group D, there was no statistically significant difference among7d,14d and21d (P>0.05). the experimental drug intervention:the second part of drug intervention group C compared with B, D form two groups observed score (see table6), joint weeks size comparison (see table7), joint synovial neutrophil number comparison (see table8), vascular number comparison in synovial joints (see table9), blood uric acid level comparison (see table10), serum XOD activity levels (see table11), the statistical analysis of7d,14d and21d difference has significant statistical significance (P<0.01). C way morphology observed score comparison (see chart6), joint weeks size comparison (see table7), joint synovial neutrophil number comparison (see table8), vascular number comparison in synovial joints (see table9), blood uric acid level comparison (see table10), serum XOD activity levels (see table11),7d and14d after statistics analysis compared with1d,14d and7d,21d,14d compare comparative difference was statistically significant (P<0.05),21d has significant difference compared with1d,7d (P<0.01).Conclusion:1. High protein, high calcium feed induce cock GA model is easy to copy, at the same time with protein content is50%and calcium content is50%when the effect is better. Section2. Juan bi Li jie cleaning has the effect of reduce the XOD of chicken GA model and the blood uric acid level, its curative effect and treatment are nearly the same as allopurinol, the mechanism of reduce GA model blood uric acid level may be associated with lower XOD levels. |
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