Experimental Study Of Acupuncture And Traditional Chinese Medicine. Ⅰ Type Hypersensitivity Model Rats

Objective：this experiment used I-type hypersensitivity sensitized animal modelfor experimental study of action mechanism of traditional Chinese medicine,system and electroacupuncture on type Ⅰ hypersensitivity, and providetheoretical basis for clinical application.Methods：the egg protein solution sensitized SD rats, resulting in typeⅠhypersensitivity reaction model. All rats were given treatment of traditionalChinese medicine, acupuncture, positive drug. Detection of IgE in serum of rats,leukotrienes (LT) content in skin tissue, IL-4, IFN-γ expression level andrats were stimulated after scratching number. Results：compared with controlgroup, the levels of serum IgE in the rats of model group increased significantly(P <0.01), scratching frequency increased (P <0.01), there was statisticalsignificance. Effect on specific IgE: the results showed that the levels of serumIgE were measured in rats of every group: compared with the blank control group,IgE of model group was significantly increased (P <0.01), the difference wasstatistically significant; compared with the model group, IgE content in serumof rats in each treatment significantly decreased (P <0.01), there wasstatistical significance; in addition to the traditional1groups, the treatmentgroup had higher IgE content than the electroacupuncture group (Chinese medicinegroup2, Chinese medicine group3, P <0.01; positive drug group P <0.05); oftraditional Chinese medicine2group,3groups of IgE were higher than oftraditional Chinese medicine1group (Chinese medicine group2P <0.05, P of3groups of traditional Chinese medicine <0.01). The effect on the expressionof IL-4, IFN-γ: compared with the blank control group, IL-4expression wasup-regulated in skin tissue model (P <0.01), IFN-γ expression levelsignificantly lowered (P <0.01), the difference was statistically significant;compared with the model group, the treatment group rat skin tissue IL-4expression levels were significantly reduced (P <0.01), significantly increasedthe expression level of IFN-γ (P <0.01), there is statistical significance; of traditional Chinese medicine2group IL-4levels were significantly higherthan that of EA group (P <0.05), there was statistical significance.③effecton serum LT content: compared with the blank control group, model group increasedsignificantly the content of leukotrienes (P <0.01), the difference wasstatistically significant; compared with the model group, the serum leukotrienecontent of rats in each treatment significantly decreased (P <0.01), there isstatistical significance; the catch number effect on scratch: the rats in controlgroup were relatively quiet, occasionally to the injection site scratching,while the rats of model group restless, increased activity, scratching frequencyincreased, the rats in the treatment group compared with the model group wasimproved to different extent, scratching frequency reduction.Conclusion:Traditional Chinese medicine1group and electroacupuncture group can beeffective in the treatment of type I hypersensitivity in rats, its mechanismmay be through regulation of Th1, Th2cytokine secretion levels, making it backto a state of balance, to prevention and treatment of type I hypersensitivitydise...