Guizhishaoyaozhimu Soup Induced Arthritis In Rats Ankle RANKL / OPG Levels In Type Ⅱ Collagen

Research background: Rheumatoid arthritis is a kind of autoimmune disease withsystemic inflammation and high rate of disabilities. It is the main disease thatmakes people lose work force and become disabled. The main cause of RA is thejoint bone destruction because of synovial inflammation and joint pannus. Itis a global disease and multiple in women of childbearing age. The attack ratioof RA is about0.4％，and0.5％～1.0％in the world. The cause of this diseaseis not clear now. It belongs to “paralysis syndromes” in traditional Chinesemedical science. According to the present research results, it is clear thatosteoclast(OC) is the main factor of Multiple joint cartilage and Bone erosionand Bone mineral density dissolved loss in the course of RA. However, theRANK/RANKL/OPG system is the important signal of differentiation and maturationand death of osteoclasts. It took part in the basic process of bone destruction.The classic prescription Guizhi Shaoyao Zhimu Decoction in Synopsis of GoldenChamber has curative effect. It is widely used in the treatment of rheumatoidarthritis. At present, the research shows Guizhi Shaoyao Zhimu can relieve theclinical symptom. But therapeutic mechanism of Guizhi Shaoyao Zhimu is unclearand we need further research.Research objective: With the pathological morphology, molecular biology andother indicators, we observe the inhibitory effect of GuizhishaoyaozhimuDecoction on CIA rat model of bone destruction. Contrasting immune inhibitorsand non-steroidal anti-inflammatory drugs, we explore the mechanism of bonedestruction.Method: This research adopts the generally accepted model of type II collageninduced arthritis rats (collagen induced arthritis CIA), switched from usingimmunosuppressant, non-steroidal anti-inflammatory drug naproxen contrast,50SD rats were randomly divided into5groups, namely Guizhi Shaoyao Zhimu highdose group, leflunomide group,naproxen group, blank control group, model group, five groups in total.Except blank control group of other4groups are in completeFreund’s adjuvant containing collagen type II sensitization after fourteenthdays, rendition, intragastric administration. Blank control group. The CIA modelgroup without any treatment. Observe the general condition of rats was recordedevery time, changes in the degree of joint swelling. In the4week afterimmunization, the rats were killed, HE staining in making left hind anklepathology observed morphological changes. With the level of expression ofRANKL/OPG in CIA rat ankle joint tissue detected by situ hybridization. explorethe mechanism of Guizhishaoyaozhimu decoction can alleviate and inhibit CIAjoint bone destruction in rats from the molecular perspective.Results:To improve the degree of arthritis, administration for2weeks, GuishaoZhimu Decoction group and naproxen and leflunomide curative effects are equal.4weeks after drug intervention, Guizhishaoyaozhimu Decoction group is betterthan the naproxen group, significant difference (P<0.01).Equal to leflunomidecurative effect, no significant difference (P>0.05), Effects of OPGmRNA,RANKLmRNA optical density value of Guizhishaoyaozhimu Decoction group is betterthan the naproxen group, significance difference (P<0.05); the effects ofleflunomide group, and Guizhishaoyaozhimu Decoction group are equal, nosignificant difference (P>0.05).