Intervention Of Aging Mice Retina Expression And Polysaccharides Of Related Genes

Objective: Observation effects of dogwood polysaccharides in the relative factorsexpression in aging model mice retina induced by D-galactose, to find the dogwoodpolysaccharides how to anti-aging in retina.Methods: Kunming mice were randomly divided into three groups, Aging modelgroup:subcutaneous injection D-galactose100mg·kg-1·d-1, Continuous45d and then by stomachinfusion warm water(0.4g·kg-1),Continuous30d. Control group: subcutaneous injectionphysiological saline100mg·kg-1·d-1, Continuous45d and then by stomach infusion warmwater(0.4g·kg-1) at10:00,Continuous30d. DOP group: subcutaneous injection D-galactose100mg·kg-1·d-1, Continuous45d and then by stomach infusion DOP (0.4g·kg-1),Continuous30d.Assay T-SOD and T-AOC in the mice retinal. Use RT-PCR and Westernblotting to assay SIRT1, FOXO1and P53in the mice retinal. Observation the influence of theabove indexes by DOP on the aging model mice.Results: Control group（341.27±10.22U/mgprot，287.40±10.70U/mgprot）have higherSOD and AOC than that of aging model group（66.92±22.74U/mgprot，104.36±6.76U/mgprot）and DOP group（78.61±27.70U/mgprot，209.44±11.11U/mgprot）（P＜0.05）. There are no difference between aging model group and DOP group,（P＞0.05）Compared with control group the expression of SIRT1mRNA and protein in the retinal of agingmodel group decline, the expression of FOXO1, P53mRNA and protein rise（P＜0.05）.The expression of SIRT1, FOXO1and P53are no difference between aging model group andDOP group（P＞0.05）.Conclusion: SIRT1, FOXO1and P53gene expression in mice retina and regulating theaging. When the body’s aging the SIRT1expression reduce and the FOXO1and P53expression increase in retinal; DOP can’t effectively influence SIRT1, FOXO1and P53geneexpression in the retina of D-galactose induced Aging model mice. When the body’s aging theT-SOD and T-AOC content reduce, DOP can’t effectively influence T-SOD and T-AOCcontent in the retina of D-galactose induced Aging model mice.