Protective Effect Of Wolfberry Chrysanthemum Granule On Rat Visual Function In Elderly Recession

ObjectiveIn this paper, used SD aged rats as a model to study the protection effect of Lycium Chrysanthemum Granules on visual function decline and to explore the related mechanisms.Methods18～22months SD rats were divided randomly into4groups:aged group, low, medium and high dose aged control group with10rats in each group. The youth blank group was grouped by10SD rats whose age were between2-3months. The low, medium and high dose aged control groups were treated with Lycium Chrysanthemum Granules once a day for Lycium Chrysanthemum Granules at lg/kg,2g/kg and4g/kg, while the other groups were treated with physiological saline solution, for35days. Determine anti-oxidation indicators (CAT, MDA) in rat heart, liver, spleen, lung, kidney and serum by colorimetric assay. Pathological section and hcmatoxylin and eosin(HE) staining to count cells in each layer of retina. Determine PEDF protein expression in rat retina and liver by Immunohistochemical assay. Analyze data by SPSS17.0statistical software.ResultsIn this paper, organism anti-oxidation was indicated by heart, liver, spleen, lung, kidney and serum. The results showed that the content of MDA of the old blank group was significantly higher than youth blank group and aged control group with low, medium and high dose (P<0.01), while the CAT activity was lower than the youth blank group and aged control group with low, medium and high dose (P<0.01). Light microscopic examination and cell counting results showed that:Compared with the elderly control group, the10retinal layer structure of the elderly high dose group and positive control group is clearer, every layer cells arranged is more compact and neat. Compared with the old blank group, the number of retinal ganglion cell (RGC) and outer nuclear layer(ONL) cells of youth blank group and aged control group with low, medium,high dose were significantly increased (P<0.01).ConclusionLycium Chrysanthemum Granules can improve CAT vitality and decrease MDA content of heart, liver, spleen, lung, kidney and serum. Thus delaying the aging which caused by free radical damage. What’s more, Lycium Chrysanthemum Granules can significantly improve the structure of rat retinal aging disorders and the reduction of layer cell number in ganglion cell layer and outer nuclear layer.