CD24, CD44 And PCNA Expression And Its Clinical Significance In Breast Cancer

Objective: To analyze the levels of expression of CD24, CD44and PCNA indifferent breast tissues by determining the levels of three markers in the tissues of breastfibroadenoma and breast cancer; To evaluate the relationship between the three makersand clinicopathologic parameters of breast cancer; To study the correlation between theirexpressions in breast cancer. Therefore, the final objective of the study is to unifydetection of expression of CD24, CD44and PCNA in breast cancer, and to analyze thevalue of CD24, CD44and PCNA in decetion of breast cancer and filtration of highfrequent patients of breast cancer.Methods: Retrospectively analyze the48cases which are the samples of modifiedradical mastectomy of breast cancer. All cases are from February2007to May2012in theAffiliated Hospital of Yan’an University. The patients are all female with mean age of48.2±8.6（32-65）years.48patients had been diagnosed with primary breast cancer thatconfirmed by pathological mechanism. All patients were not accepted radiotherapy,chemotherapy and endocrine therapy before surgery. In addition,30cases were fromtissues of breast fibroadenoma patients, which accepted surgery and confirmed bypathological mechanism in the Affiliated Hospital of Yanan University. By S-Pimmunohistochemical technique, the expression of CD24, CD44and PCNA wereinvestigated in different tissues of breast fibroadenoma and breast cancer. Statisticalmethods: chi-square test and Spearman rank correlation analysis were used (SPSSstatistical software, Version13.0for windows).Results: The positive signals of CD24and CD44express in cytomembrane andcytoplasm. The positive signal of PCNA express in cell nucleus. In the tissues of breastmaliqnancy, the decetion of CD44were30cases in48cases (62.5%), CD24were32cases in48cases (66.7%), PCNA were33cases in48cases (68.8%). In the30cases ofthe tumor-surrounding tissue of breast fibroadenoma, the positive expression of CD24were9cases (30.0%), which of CD44were7cases (23.3%), that of PCNA were10cases(33.3%). With respect to the positive expression of CD24, CD44and PCNA, there was significant difference between breast cancer and the tumor-surrounding tissue of breastfibroadenoma (P＜0.05). The positive expressive rates of CD44and PCNA in patientswith breast cancer were significantly correlated with axillary lymph node status andhistological grading (P＜0.05). While no significant correlation with the age of patients,the size of tumor and TNM staging (P＞0.05). The positive expressive rates of CD44andPCNA were62.5%and68.8%respectively for patients with axillary lymph nodemetastasis, significantly higher than those without metastasis (31.6%,42.1%respectively); The positive expressive rates of CD44and PCNA in the histologicalgradingⅠof breast cancer were27.3%and36.4%respectively, which is lower than thosein histological gradingⅡ+Ⅲ of breast cancer (73.0%,75.7%respectively). In addition,the result of this study is that there was a positive correlation between two markers of theexpression of CD24, CD44, PCNA in the tissues of breast cancer (P＜0.05).Conclusion: The expression of CD24, CD44and PCNA may be related to thecarcinogenesis and progression of breast cancer. The positive expression of CD24, CD44and PCNA were correlated with the tissues of breast cancer in the study. In this study, theexpression of CD44and PCNA were correlated with histological grade and metastasis oflymph node. The expression of CD24, CD44and PCNA might be useful indicators toevaluate early diagnosis and biological behaviors, and could be considered as a goodearly diagnosis, treatment and predictor for breast cancer. There was a positivecorrelation between the expression of CD24, CD44and PCNA in breast cancer, whichcan provide clinical significance in diagnosis of breast cancer and filtration of highfrequent patients of breast cancer by combining detections of CD24, CD44and PCNA.