Preparation Of Chitosan Studies Of Europium With Fluorescent Material

Rare earth and chitosan both have excellent physiological activities. Rare earth has the properties of sterilization, anticancer, anticoagulation and so on. Chitosan is biodegradable, non-toxic and biocompatible. The synthesis of europium modified chitosan fluorescent material is the primary objective of this paper and then explored its preliminary application in the area of drug concentration detection.1. Synthesis and properties of the complexes containing Eu3+The complexes containing Eu3+were prepared with europium oxide, methyl acrylic acid and1,10-phenanthroline as raw materials. The structure and composition information of the complexes were got through the infrared absorption spectroscopy (IR) and ultraviolet absorption spectroscopy (UV). The methods of x-ray powder diffraction pattern (XRD) and field emission scanning electron microscopy (FESEM) were used to study the crystallinity and morphology of the complexes. The thermal stability of the complexes was explored by simultaneous thermal analysis (STA). Then the fluorescent property of the complexes containing Eu3+was discussed by the fluorescence spectrometer.2. Synthesis and characterization of europium modified chitosan fluorescent materialThe fluorescent material of rare earth modified chitosan was synthesized through the method of radical polymerization with ammonium persulfate (APS) as the initiator, acetic acid (HOAc) and dimethylsulfoxide (DMSO) as the solvents. The rare earth complex Eu (MAA)3phen which is polymerisable, MAA and chitosan were the raw materials of the reaction. A series of rare earth modified chitosan fluorescent materials were prepared by changing the amount of the complex, acetic acid and initiator added into the reaction.Methods of infrared absorption spectroscopy (IR), ultraviolet absorption spectroscopy (UV), x-ray powder diffraction pattern (XRD) and the simultaneous thermal analysis (STA) were used to study the form of Eu (MAA)3phen existed in the copolymer and the thermal stability of the product. The results showed that the rare earth complex and chitosan were combined via chemical bonds. The morphology of the rare earth modified chitosan fluorescent material was observed by the field emission scanning electron microscopy (FESEM). With the help of inductively coupled plasma mass spectrometry (ICP-MS), the Eu percentage contents of series of rare earth modified chitosan materials were obtained. At last, the fluorescence property of the copolymers was studied through fluorescence spectrometer. Then the relationship between the fluorescent intensity of the products with the mass change of Eu (MAA)3phen, glacial acetic acid and the initiator was discussed.3. The explorations of the fluorescent systems consisting of the rare earth modified chitosan fluorescent material and quinoloneThe two co-luminescence systems consisting of rare earth modified chitosan fluorescent material and sparfloxacin/ciprofloxacin were optimized by their fluorescence intensities. Under the optimum conditions, the relationship between the fluorescence intensity of the systems with the concentration of quinolone was studied. The results showed that both the two drugs played a role in the enhancement of the fluorescence intensities of the systems. And two good linear relationships were got.The significance of the subject is the synthesis of bonded rare earth modified chitosan fluorescent material which is quite different from rare earth chitosan complexes combined by coordination bond as reported. Due to the bond of the rare earth complex combined with chitosan, the chromophores of the complex would not fall off easily and was distributed uniform. The rare earth could also be more stable during the application process of the product. Thus the fluorescent property and physiological activity of rare earth modified chitosan material would be more stable and excellent.