Study On Chitosan-graft Copolymer And Target Ing Drug Release Material Preparation And Properties

Targeting sustained-release pharmaceutical preparations with fast positioning and efficient drug delivery,can conquer the drawback of traditional medicines and better meet the clinical needs.Chitosan has been attracted much attention by science researcher for its non-toxic,excellent antibiosis,biodegradability,biocompatibility and rich resources.The water insolubility of chitosan limit its application,therefore,chitosan must be modified to derivatives to enhance its solubility and give it new functions in order to extend range of applications.In this paper,the research progress of chitosan graft modification and its application was summarized,two kinds of graft copolymers were prepared.property of the material of graft copolymer and magnetic nanoparticl was investigated for protein drugs loading and release.The main contents and results of the paper are explained as follows:1.chitosan(CTS)and vinyl acetate(VAc)as materials,ammonium persulfate(APS)as initiator initiated the grafting copolymerization reaction.The dosage of monomer,concentration of initiator,reaction temperature and reaction time were investigated on the graft copolymerization.The copolymer was characterized by FT-IR,SEM,XRD and TG.The results of orthogonal experiment showed the optimum reaction conditions were 2.0 g mass of CTS,an addition of 7 g of monomer,0.7 g/L of concentration of(NH4)2S2O8,65℃ of reaction temperature,4 h of reaction time.Under this condition the graft yield and graft efficiency of the copolymer were 163%and 49%,respectively.The FT-IR,SEM,XRD and TG revealed the chitosan was modified with vinyl acetate successfully.2.CTS and 2-tert-butylaminoethyl methacrylate(TBAEMA)as material s,ammonium persulfate and sodium sulphite as redox initiator initiated t he grafting copolymerization.the dosage of monomer,concentration of init iator,the ratio of redox intiator,reaction temperature and reaction time w ere investigated on the graft copolymerization.The copolymer was charact erized by FT-IR,SEM,XRD and TG.Moreover,the antibacterial activity of copolymer was tested for inhibiting escherichia coli and staphylococcu s aureus.The results of orthogonal experiment showed the optimum reacti on conditions were 2.0 g mass of CTS,an addition of 12 g of monomer,3 g/L of concentration of intiator,2:1 of the ratio of redox intiator,60℃ of reaction temperature,8 h of reaction time.Under this condition the graft yield and graft efficiency of the copolymer were 163%and 49%,respectively.Antibacterial experiment results revealed the copolymer have the excellent bacteriostatic action for inhibiting escherichia coli and staph ylococcus aureus.The MIC of copolymer for inhibiting escherichia coli a nd staphylococcus aureus was 0.04%and 0.02%,respectively.The FT-IR,SEM,XRD and TG.displayed the chitosan was modified with 2-tert-butyl aminoethyl methacrylate successfully.3.Graft copolymer,magnetic Fe3O4 nanoparticles as Targeted drugs slow-release materials,the films and the tablets were perpared by using tape casting and low temperature grinding tableting,respectively.The films and tablets have ave the excellent performance for loading and bovine serum albumin(BSA)which as the model medicine,and then the drugs release performance was investigated under the simulated biological environment.The result of release experiment showed that:the material have the well release performance in vitro.the BSA release percent and release time of the film which is made of grafted rate is 98%of the CTS-g-VAc copolymer is 89.8%and 10 h,respectively.The BSA release percent and release time of the film which is made of grafted rate is38%of the CTS-g-TBAEMA copolymer is 91%and 9 h,respectively.the BSA release percent of CTS-g-VAc copolymer tablet is 95%and the release time of this tablet is 9 h.The BSA release percent of CTS-g-TBAEMA copolymer tablet is 98%,and the release time of this tablet was 11 h.