S. Pombe Study Involved Methylglyoxal Degradation Pathway Related Gene Function

This topic mainly consists of two parts:one is the study of methylglyoxal biodegradation pathway genes, the other is with the human prostate cancer related gene Sptrz2genetic screening of genetic interactions.As is known to all, glycolytic pathway is the process of the body produce energy, but of which the metabolite pyruvic aldehyde are toxic to cells, can lead to many diseases, acetone aldehyde in the way of dissolving a subject many scientists are interested in. Glyoxalase system has glyoxalase I and glyoxalase II constitute, the former relies on glutathione methylglyoxal degradation as S-D-lactic acid, the latter is the degradation of the product sul phur ester, S-D-lactic acid can be converted to lactic acid and glutathione. SpGlolp are widespread in the degradation pathways of acetone aldehyde, under oxygen stress, genes SpGlol regμl ated against poison.This topic first by using the principle of homologous arm restructuring build missing Spglol gene of yeast strains, by controlling the acetone aldehyde concentration in the medium to study the changes in phenotype, and explore its role under the condition of high oxygen stress. Were determined and the growth curve, according to the above data we obtained Spglol genes play a major role in the digestion methylglyoxal way. This strain is conducive to continue to explore Spglol interactions of genes, providing theoretical basis for medicine from the viewpoint of molecμl ar biology.In Schizosaccharomyces pombe there exists two tRNase Z genes, designated sptrzl (SPAC1D4.10) and sptrz2(SPBC3D6.03c), encoding the nuclear and mitochondrial tRNase Zs, respectively. In our previous study, we have demonstrated that both of the two tRNase Zs in Schizosaccharomyces pombe are essential. In this study,we have got a ts strain of sptrz2and carried out studies on the function of it. To know whether there are proteins in vovo that functionally complement with SpTrz2p, we carried out genetic studies by selecting high copy repressor genes of the sptrz2-A623V mutation from cDNA libraries, namely,SPLE1. To explore the method of improving the efficiency of conversion from yeast. But based on all aspects of the reasons for failed to succeed. So we choose through reference individual related gene cloning, trying to save, and finally get pWH5series plasmids can be very good save sptrz2temperature sensitive mutant strain. On this basis, we adopt the method of continuous truncation upstream trz2gene sequences to explore its promoter regions.