Qingfei Detoxification Decoction Effect On Streptococcus Pneumoniae, Klebsiella Pneumoniae Two Lung Of Aged Pneumonia Rat TNF Alpha, IFN-Gamma, IL-6and IL-10Content Changes

Objective:Through the establishment of pneumococcal pneumonia withpneumonia klebsiella pneumonia bacteria pneumonia in aged rats with two model, afterthe experimental observation clear lung detoxification of streptococcus pneumoniae andtwo elderly pneumonia klebsiella pneumonia bacteria inflammation of lung tissuehomogenate of rats factor TNF alpha, IFN-gamma, IL-6, IL-10levels change, use ofclearing lung detoxification drinks on the two kinds of pneumonia in rats, comparedbefore and after the change of the index of pneumonia, dosing to drink clear lungdetoxification treatment provide experimental basis for two kind of pneumonia.Methods:Chooses SD aging rats20to22months, weight of560g and90g,140(SPF) only SD aging male rats were randomly divided into7groups, respectively inaged rats with normal group, streptococcus pneumoniae, model group, klebsiellapneumoniae, aging model group and two kinds of model rats therapy of clearing lungdetoxification drink high and low dose group (hereinafter referred to as the lungs chainhigh and low dose group and lung grams of high and low dose group). Each group of20.Adaptive feed for a week. Methods of reference Yershov, respectively streptococcuspneumoniae, klebsiella pneumoniae, endotracheal injection. Will be10%chloralhydrate produced by intraperitoneal injection of0.35mL/100g make anesthesia in rats,and the middle incisor point suspension, with a pick up syringes blunt needle insertedthrough the mouth trachea, drip into the40mu contains about107cfu L respectively inthe logarithmic phase of streptococcus pneumoniae, klebsiella pneumoniae suspensionliquid (in trendy blocked nose drops), make the inoculum into the lungs. Acutepneumonia induced successfully soon.According to the above method model after intragastric administration, eachdosage according to body weight of rats in a dose conversion of adult rats [according tothe formula of equivalent dose (mg/kg)=x adult dose conversion coefficients (mg/kg)]to calculate the equivalent dose for rat: Traditional Chinese medicine:8.19g/kg, high, low dose group of Chinese medicine were given medicine16.4,4.10g/(kg· d) bygavage, the normal group and two in aged rats with model group respectively filled withsaline. Continuous administration for14days, then the rats were killed. The chestradiograph, check the rat laboratory examination and immunohistochemical changes.Use of the first affiliated hospital of anhui medical university clinical commonrespiratory Qingfei Detoxification Decoction treatment prescriptions, observation ofclearing lung detoxification drink for two kinds of pneumonia in the rat lung tissuehomogenate inflammatory factor TNF alpha, IFN-gamma, IL-6, the change of thecontent of IL-10, compared to pneumonia index before and after the change.Results:ELISA analysis, according to the results of pneumococcal pneumonia inrats after treatment within14days after high dose group of the lung tissue of TNF alpha,IFN-gamma, IL-6, IL-10significantly lower than the model group, with statisticalsignificance (P <0.01). Klebsiella pneumoniae pneumonia in rats after treatment within14days after high dose group of the lung tissue of TNF alpha, IFN-gamma, IL-6, IL-10significantly lower than the model group, with statistical significance (P <0.01).Two kinds of pneumonia rats between lower dose treatment groups and itscorresponding model group has no statistical significance.Conclusion: Qingfei Decoction on the effective dose of Streptococcus pneumoniae,Klebsiella pneumoniae pneumonia in rats of two kinds of treatment, the regulatory roleof cytokines.